Correlation of interleukin-6 and monocyte chemotactic protein-1 concentrations with crescent formation and myeloperoxidase-specific anti-neutrophil cytoplasmic antibody titer in SCG/Kj mice by treatment with anti-interleukin-6 receptor antibody or mizoribine.
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| Abstract |
Myeloperoxidase-specific anti-neutrophil cytoplasmic antibody (MPO-ANCA) is associated with rapidly progressive glomerulonephritis (RPGN) and glomerular crescent formation. Pathogenic factors in RPGN were analyzed by using SCG/Kj mice, which spontaneously develop MPO-ANCA-associated RPGN. The serum concentration of soluble IL-6R was determined by using ELISA and those of another 23 cytokines and chemokines by Bio-Plex analysis. Sections of frozen kidney tissue were examined by fluorescence microscopy and the CD3(+) B220(+) T cell subset in the spleen determined by a flow cytometry. Concentrations of IL-6 and monocyte chemotactic protein-1 were significantly correlated with the percentages of crescent formation. Anti-IL-6R antibody, which has been effective in patients with rheumatoid arthritis, was administered to SCG/Kj mice to elucidate the role of IL-6 in the development of RPGN. MPO-ANCA titers decreased after administration of anti-IL-6R antibody, but not titers of mizoribine, which is effective in Kawasaki disease model mice. These results suggest that IL-6-mediated signaling is involved in the production of MPO-ANCA.
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| Authors | Tomokazu Nagao, Reina Kusunoki, Chiaki Iwamura, Shigeto Kobayashi, Wako Yumura, Yosuke Kameoka, Toshinori Nakayama, Kazuo Suzuki |
| Journal | Microbiology and immunology (Microbiol Immunol) Vol. 57 Issue 9 Pg. 640-50 (Sep 2013) ISSN: 1348-0421 [Electronic] Australia |
| PMID | 23829825 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © 2013 The Societies and Wiley Publishing Asia Pty Ltd. |
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