Abstract | AIMS: METHODS AND RESULTS: A total of 130 formalin-fixed and paraffin-embedded gastric tumour samples with corresponding normal gastric and tumour-adjacent tissues were used. High levels of GRP94 expression (2+ or 3+) were observed in 109 of 130 gastric carcinomas (83.85%) by immunohistochemistry, and in 13 of 18 tumour specimens (72.22%) with Western blot analysis. GRP94 expression was correlated positively with gender, tumour stage, lymph node metastasis and ER-α36 expression (P < 0.05). Oestrogen treatment up-regulated both GRP94 and ER-α36 expression in gastric cancer SGC7901 cells. In addition, steady state levels of GRP94 protein were decreased in established gastric cancer SGC7901 cells with knocked-down levels of ER-α36 expression and in xenograft tumours formed by these cells. Forced expression of recombinant ER-α36 in SGC7901 cells, however, up-regulated the levels of GRP94 expression. CONCLUSIONS:
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Authors | Zhengqi Fu, Hao Deng, Xuming Wang, Xiuping Yang, Zhaoyi Wang, Lijiang Liu |
Journal | Histopathology
(Histopathology)
Vol. 63
Issue 3
Pg. 325-33
(Sep 2013)
ISSN: 1365-2559 [Electronic] England |
PMID | 23829397
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 John Wiley & Sons Ltd. |
Chemical References |
- ESR1 protein, human
- Estrogen Receptor alpha
- Membrane Glycoproteins
- endoplasmin
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Topics |
- Adenocarcinoma
(etiology, metabolism, pathology)
- Adult
- Aged
- Aged, 80 and over
- Animals
- Blotting, Western
- Carcinogenesis
- Cell Line, Tumor
- Estrogen Receptor alpha
(antagonists & inhibitors, genetics, metabolism)
- Female
- Gene Knockdown Techniques
- Heterografts
- Humans
- Immunohistochemistry
- Lymphatic Metastasis
- Male
- Membrane Glycoproteins
(metabolism)
- Mice
- Mice, Nude
- Middle Aged
- Neoplasm Staging
- Signal Transduction
- Stomach Neoplasms
(etiology, metabolism, pathology)
- Up-Regulation
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