Abstract |
We have evaluated the in vitro activity of caspofungin against 36 wild-type strains of Candida parapsilosis sensu stricto using 3 techniques: broth microdilution, disk diffusion, and the determination of minimal fungicidal concentration (MFC). The first 2 methods showed a good in vitro activity of caspofungin, but the MFCs were ≥2 dilutions above their corresponding MICs. In a murine model of disseminated infection, we evaluated the efficacy of caspofungin at 5 mg/kg against 8 strains of C. parapsilosis representing different degrees of in vitro susceptibility (0.12-1 μg/mL). All the isolates responded to treatment and (1→3)-β-D-glucan levels were reduced in all the cases; however, the study revealed differences among isolates, since caspofungin reduced the tissue burden of mice infected with isolates with MICs ≤0.5 μg/mL but was less effective against those with MICs of 1 μg/mL.
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Authors | Valentina Salas, F Javier Pastor, Javier Capilla, Deanna A Sutton, Emilio Mayayo, Annette W Fothergill, Michael G Rinaldi, Josep Guarro |
Journal | Diagnostic microbiology and infectious disease
(Diagn Microbiol Infect Dis)
Vol. 77
Issue 1
Pg. 41-5
(Sep 2013)
ISSN: 1879-0070 [Electronic] United States |
PMID | 23806662
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Echinocandins
- Lipopeptides
- Proteoglycans
- beta-Glucans
- polysaccharide-K
- Caspofungin
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Topics |
- Animals
- Candida
(drug effects)
- Candidiasis
(drug therapy, microbiology)
- Caspofungin
- Colony Count, Microbial
- Disease Models, Animal
- Echinocandins
(pharmacology, therapeutic use)
- Lipopeptides
- Male
- Mice
- Microbial Sensitivity Tests
- Proteoglycans
- Treatment Outcome
- beta-Glucans
(blood)
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