[Effects of T-2 toxin of Fusarium on proliferation and apoptosis of human acute promyelocytic leukemia cell line HL60].

Abstract

OBJECTIVE:

To study the effects of sesquiterpenes compounds T-2 toxin produced by Fusarium fungi on proliferation and apoptosis of acute promyelocytic leukemia cellline HL60.

METHODS:

HL60 cells were treated with T-2 toxin (0, 4, 8, 16 and 32 microg/ml) for 48 h, and then cells were harvested for the studies of growth inhibition with MTT, Cell morphology with Giemsa staining, apoptosis and the expression of Bax and Bcl-2 with flowcytometry (FCM) and Western blot.

RESULTS:

MTT results showed that T-2 toxin inhibited HL60 proliferation on dose-dependent. Morphological observation showed significant apoptotic morphological characteristics. FCM results showed that the apoptosis rates of HL60 cells in T-2 toxin treatment groups were higher than that in control, and positive dose-effect correlations could be found. FCM and Western blot results showed that the expression of Bax was increased while that of Bcl-2 was decreased in T-2 toxin treatment groups of HL60.

CONCLUSION:

The results suggested that T-2 toxin could inhibite proliferation and induce apoptosis of HL60 cells in vitro in a dose-dependent manner.

• Authors: Yiju Hou, Zhonghai Yuan, Wei Guo, Huiyan Wang
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 42 Issue 3 Pg. 381-5 (May 2013) ISSN: 1000-8020 [Print] China
• PMID: 23805511 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BAX protein, human
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• T-2 Toxin

Topics

• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Dose-Response Relationship, Drug
• HL-60 Cells
• Humans
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• T-2 Toxin (pharmacology)
• bcl-2-Associated X Protein (metabolism)