T85C polymorphisms of the dihydropyrimidine dehydrogenase gene detected in gastric cancer tissues by high-resolution melting curve analysis.

Abstract

BACKGROUND/AIMS:

Dihydropyrimidine dehydrogenase is a key enzyme acting on the metabolic pathway of medications for gastric cancer. High-resolution melting curve technology, which was developed recently, can distinguish the wild-type dihydropyrimidine dehydrogenase gene from multiple polymorphisms by fluorescent quantitative polymerase chain reaction products in a direct and effective manner.

MATERIALS AND METHODS:

T85C polymorphisms of dihydropyrimidine dehydrogenase in the peripheral blood of 112 Chinese gastric cancer patients were detected by real-time polymerase chain reaction combined with high-resolution melting curve technology. Primer design, along with the reaction system and conditions, was optimized based on the GenBank sequence.

RESULTS:

Seventy nine cases of wild-type (TT, [70.5%]), 29 cases of heterozygous (TC, [25.9%]), and 4 cases of homozygous mutant (CC, [3.6%]) were observed. The result was completely consistent with the results of the sequencing.

CONCLUSIONS:

Real-time polymerase chain reaction combined with high-resolution melting curve technology is a rapid, simple, reliable, direct-viewing, and convenient method for the detection and screening of polymorphisms.

• Authors: Weijia Fang, Nong Xu, Dazhi Jin, Yu Chen, Xiaogang Chen, Yi Zheng, Hong Shen, Ying Yuan, Shusen Zheng
• Journal: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology (Turk J Gastroenterol) Vol. 23 Issue 6 Pg. 652-7 ( 2012) ISSN: 2148-5607 [Electronic] Turkey
• PMID: 23794300 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't, Validation Study)

Chemical References

• Dihydrouracil Dehydrogenase (NADP)

Topics

• Adult
• Asian People (genetics)
• Dihydrouracil Dehydrogenase (NADP) (genetics)
• Female
• Genetic Predisposition to Disease (genetics)
• Genetic Testing (methods, standards)
• Heterozygote
• Homozygote
• Humans
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Real-Time Polymerase Chain Reaction (methods, standards)
• Reproducibility of Results
• Stomach Neoplasms (genetics)