Performances of malaria P.f/Pan rapid test device Acon® (Pf HRP2/pan aldolase) and malaria Pf rapid test device Acon® (Pf HRP2) for the diagnosis of malaria in adults and children living in Gabon, Central Africa.

Abstract

The Malaria Pf Rapid Test Device Acon® (Acon Labs) and the pan HRP2/aldolase RDT, Malaria P.f/Pan Rapid Test Device Acon® (Acon Labs), performances were evaluated for malaria species diagnosis in 592 febrile patients living in Gabon using microscopy as gold standard. Sensitivities were equal or above 96.0% for Plasmodium falciparum detection, of 62.5% for non-P. falciparum malaria species detection and higher in younger children (100%). Negative predictive values were greater than 97.0%. Acon®HRP2 had a higher specificity (96.6%) and lower false-positive (FP) rate (9.3%) compared to Acon®Pf/Pan, which had a specificity of 87.3% and a FP rate of 27.1% (P < 0.01). Overall, 32.5% of all Acon® Pf/Pan tests resulted in a "faint band" with only 2 resulted from samples with a parasitemia below 100 p/μL. The accuracy of Acon®HRP2 RDT for the diagnosis of P. falciparum infection is confirmed. However, the high FP rate observed with Acon®Pf/Pan is a limitation for its use.

• Authors: Marielle Karine Bouyou Akotet, Denise Patricia Mawili-Mboumba, Blondel Madoungou, Maryvonne Kombila
• Journal: Diagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 77 Issue 1 Pg. 58-63 (Sep 2013) ISSN: 1879-0070 [Electronic] United States
• PMID: 23791385 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Topics

• Adolescent
• Adult
• Child
• Child, Preschool
• Clinical Laboratory Techniques (methods)
• Female
• Gabon
• Humans
• Immunoassay (methods)
• Malaria, Falciparum (diagnosis)
• Male
• Middle Aged
• Parasitology (methods)
• Plasmodium falciparum (immunology)
• Point-of-Care Systems
• Predictive Value of Tests
• Sensitivity and Specificity
• Young Adult