Abstract | BACKGROUND: The renal distal tubule fine-tunes renal epithelial calcium transport. Dietary intake of salt and fluid varies day-to-day and the kidney adapts accordingly to maintain homeostasis. The alternations in salt and fluid balance affect calcium and magnesium transport in the distal tubule, but the mechanisms are not fully understood. METHODS: Sprague-Dawley rats were grouped into high- salt, low- salt and dehydration treatment. Daily intake, water consumption and urine output were recorded. At the end of the experiment, blood and urine samples were collected for hormonal and biochemical tests. Genetic analysis, immunoblotting and immunofluorescence studies were then performed to assess the alterations of calcium and magnesium transport-related molecules. RESULTS: CONCLUSIONS:
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Authors | Chien-Te Lee, Yeong-Hau H Lien, Li-Wen Lai, Hwee-Yeong Ng, Terry Ting-Yu Chiou, Hung-Chun Chen |
Journal | Nephron. Physiology
(Nephron Physiol)
Vol. 122
Issue 3-4
Pg. 19-27
( 2012)
ISSN: 1660-2137 [Electronic] Switzerland |
PMID | 23774784
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 S. Karger AG, Basel. |
Chemical References |
- Calbindin 1
- Calcium Channels
- Parathyroid Hormone
- Sodium Chloride, Dietary
- TRPM Cation Channels
- TRPM6 protein, rat
- TRPV Cation Channels
- Sodium
- Magnesium
- Calcium
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Topics |
- Animals
- Calbindin 1
(genetics, metabolism)
- Calcium
(urine)
- Calcium Channels
(metabolism)
- Drinking
- Gene Expression Regulation
- Kidney Tubules, Distal
(metabolism)
- Magnesium
(metabolism, urine)
- Male
- Parathyroid Hormone
(blood)
- Rats
- Rats, Sprague-Dawley
- Sodium
(urine)
- Sodium Chloride, Dietary
(administration & dosage, metabolism)
- TRPM Cation Channels
(genetics, metabolism)
- TRPV Cation Channels
(genetics, metabolism)
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