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Synthetic phosphoethanolamine induces cell cycle arrest and apoptosis in human breast cancer MCF-7 cells through the mitochondrial pathway.

Abstract
Phosphoethanolamine (Pho-s) is a compound involved in phospholipid turnover, acting as a substrate for many phospholipids of the cell membranes. In a recent study, we showed that Pho-s has antitumor effect in the several tumor cells. In this study we evaluated the antitumor activity of synthetic Pho-s on MCF-7 breast cancer cells. Here we demonstrate that Pho-s is cytotoxic to MCF-7 cells in a dose-dependent manner, while it is cytotoxic to MCF10 only at higher concentrations. In addition, Pho-s induces a disruption in mitochondrial membrane potential (Δψm). Furthermore, Pho-s induces mitochondria aggregates in the cytoplasm and DNA fragmentation of MCF-7 cells visualized by confocal microscopy. In agreement with the reduction on Δψm, we showed that Pho-s induces apoptosis followed by an increase in cytochrome c expression and capase-3-like activity in MCF-7 cells. Our results demonstrate that Pho-s induces a cell cycle arrest in the G1 phase through an inhibition of cyclin D1 and stimulates p53. An additional highlight of this study is the finding that Pho-s inhibits Bcl-2, inducing apoptosis through the mitochondrial pathway. Taken together, these results show that Pho-s is a promising compound in the fight against cancer.
AuthorsAdilson Kleber Ferreira, Renato Meneguelo, Alexandre Pereira, Otaviano Mendonça R Filho, Gilberto Orivaldo Chierice, Durvanei Augusto Maria
JournalBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 67 Issue 6 Pg. 481-7 (Jul 2013) ISSN: 1950-6007 [Electronic] France
PMID23773853 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Masson SAS. All rights reserved.
Chemical References
  • Antineoplastic Agents
  • Ethanolamines
  • Proto-Oncogene Proteins c-bcl-2
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • Cyclin D1
  • phosphorylethanolamine
  • Cytochromes c
  • Caspase 3
Topics
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects, genetics)
  • Breast Neoplasms (drug therapy, genetics, metabolism)
  • Caspase 3 (genetics, metabolism)
  • Cell Cycle Checkpoints (drug effects, genetics)
  • Cell Death (drug effects, genetics)
  • Cell Line, Tumor
  • Cyclin D1 (genetics, metabolism)
  • Cytochromes c (genetics, metabolism)
  • Cytoplasm (drug effects, genetics, metabolism)
  • DNA Fragmentation (drug effects)
  • Ethanolamines (pharmacology)
  • Female
  • G1 Phase (drug effects, genetics)
  • Humans
  • MCF-7 Cells
  • Membrane Potential, Mitochondrial (drug effects, genetics)
  • Mitochondria (drug effects, genetics, metabolism)
  • Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
  • Signal Transduction (drug effects, genetics)
  • Tumor Suppressor Protein p53 (genetics, metabolism)

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