Altered hepatic gene expression profiles associated with improved fatty liver, insulin resistance, and intestinal permeability after hydroxypropyl methylcellulose (HPMC) supplementation in diet-induced obese mice.

The effect of hydroxypropyl methylcellulose (HPMC) on hepatic gene expression was analyzed by exon microarray and real-time PCR from livers of diet-induced obese (DIO) mice fed a high-fat (HF) diet supplemented with either 6% HPMC or 6% microcrystalline cellulose (MCC). HPMC-fed mice exhibited significantly reduced body weight gain (55% lower compared to MCC), liver weight (13%), plasma LDL-cholesterol concentration (45%), and HF diet-increased intestinal permeability (48%). HPMC significantly reduced areas under the curve for 2 h insulin and glucose responses, indicating enhanced insulin sensitivity and glucose metabolism. HPMC up-regulated hepatic genes related to fatty acid oxidation, cholesterol and bile acid synthesis, and cellular activation of glucocorticoid (bile acid recycling) and down-regulated genes related to oxidative stress, triglyceride synthesis, and polyunsaturated fatty acid elongation. In conclusion, HPMC consumption ameliorates the effects of a HF diet on intestinal permeability, insulin resistance, hepatic lipid accumulation, glucocorticoid-related bile acid recycling, oxidative stress, and weight gain in DIO mice.
AuthorsHyunsook Kim, Glenn E Bartley, Scott A Young, Kun-Ho Seo, Wallace Yokoyama
JournalJournal of agricultural and food chemistry (J Agric Food Chem) Vol. 61 Issue 26 Pg. 6404-11 (Jul 03 2013) ISSN: 1520-5118 [Electronic] United States
PMID23742138 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Prebiotics
  • Hypromellose Derivatives
  • Cellulose
  • Methylcellulose
  • microcrystalline cellulose
  • Animals
  • Cellulose (therapeutic use)
  • Diet, High-Fat (adverse effects)
  • Fatty Liver (etiology, prevention & control)
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Hypromellose Derivatives
  • Insulin Resistance
  • Intestinal Absorption
  • Liver (enzymology, metabolism)
  • Methylcellulose (analogs & derivatives, therapeutic use)
  • Mice
  • Non-alcoholic Fatty Liver Disease
  • Obesity (diet therapy, etiology, metabolism, physiopathology)
  • Prebiotics

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!

Choose Username:
Verify Password:
Enter Code Shown: