Structure elucidation and inhibitory effects on human platelet aggregation of chlorogenic acid from Wrightia tinctoria.

Abstract

BACKGROUND:

Interest in natural compounds as sources of potentially new treatment options is growing rapidly. Preliminary screening of many different plant extracts showed that Wrightia tinctoria acts as a potent human platelet aggregation inhibitor. The aim of the present study was to isolate and characterize the active compound responsible for potent inhibition of human platelet aggregation in vitro.

METHODS:

A 70% ethanolic extract derived from W. tinctoria seeds was fractionated with chloroform followed by ethyl acetate. The ethyl acetate fraction was further fractionated and purified through a series of three successive column chromatographic separations using silica gel, Sephadex LH-20, and C-18 columns. Liquid chromatography coupled to negative electrospray ionization tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) studies were performed in the structure determination of the active phenolic compound present in the ethyl acetate fraction of W. tinctoria seeds.

RESULTS:

A phenolic compound has been isolated and identified as chlorogenic acid by LC-MS/MS and NMR studies. Chlorogenic acid showed concentration-dependent inhibitory effect on collagen-induced platelet aggregation in vitro with an IC50 of 0.2363 μg/μl.

CONCLUSION:

The present data suggest that chlorogenic acid can be developed as potential antiplatelet agent in the treatment of cardiovascular diseases in diabetes mellitus.

• Authors: Ripal P Amin, Naveen Kunaparaju, Sunil Kumar, Tony Taldone, Michael A Barletta, S William Zito
• Journal: Journal of complementary & integrative medicine (J Complement Integr Med) Vol. 10 (May 24 2013) ISSN: 1553-3840 [Electronic] Germany
• PMID: 23735478 (Publication Type: Journal Article)

Chemical References

• Plant Extracts
• Platelet Aggregation Inhibitors
• Chlorogenic Acid
• Collagen

Topics

• Apocynaceae (chemistry)
• Chlorogenic Acid (chemistry, isolation & purification, pharmacology)
• Collagen (metabolism)
• Dose-Response Relationship, Drug
• Humans
• Inhibitory Concentration 50
• Molecular Structure
• Plant Extracts (chemistry, pharmacology)
• Platelet Aggregation (drug effects)
• Platelet Aggregation Inhibitors (chemistry, isolation & purification, pharmacology)