Transcript expression of vesicular glutamate transporters in lumbar dorsal root ganglia and the spinal cord of mice - effects of peripheral axotomy or hindpaw inflammation.

Abstract

Using specific riboprobes, we characterized the expression of vesicular glutamate transporter (VGLUT)₁-VGLUT₃ transcripts in lumbar 4-5 (L4-5) dorsal root ganglions (DRGs) and the thoracolumbar to lumbosacral spinal cord in male BALB/c mice after a 1- or 3-day hindpaw inflammation, or a 7-day sciatic nerve axotomy. Sham animals were also included. In sham and contralateral L4-5 DRGs of injured mice, VGLUT₁-, VGLUT₂- and VGLUT₃ mRNAs were expressed in ∼45%, ∼69% or ∼17% of neuron profiles (NPs), respectively. VGLUT₁ was expressed in large and medium-sized NPs, VGLUT₂ in NPs of all sizes, and VGLUT₃ in small and medium-sized NPs. In the spinal cord, VGLUT₁ was restricted to a number of NPs at thoracolumbar and lumbar segments, in what appears to be the dorsal nucleus of Clarke, and in mid laminae III-IV. In contrast, VGLUT₂ was present in numerous NPs at all analyzed spinal segments, except the lateral aspects of the ventral horns, especially at the lumbar enlargement, where it was virtually absent. VGLUT₃ was detected in a discrete number of NPs in laminae III-IV of the dorsal horn. Axotomy resulted in a moderate decrease in the number of DRG NPs expressing VGLUT₃, whereas VGLUT₁ and VGLUT₂ were unaffected. Likewise, the percentage of NPs expressing VGLUT transcripts remained unaltered after hindpaw inflammation, both in DRGs and the spinal cord. Altogether, these results confirm previous descriptions on VGLUTs expression in adult mice DRGs, with the exception of VGLUT₁, whose protein expression was detected in a lower percentage of mouse DRG NPs. A detailed account on the location of neurons expressing VGLUTs transcripts in the adult mouse spinal cord is also presented. Finally, the lack of change in the number of neurons expressing VGLUT₁ and VGLUT₂ transcripts after axotomy, as compared to data on protein expression, suggests translational rather than transcriptional regulation of VGLUTs after injury.

• Authors: M Malet, C A Vieytes, K H Lundgren, R P Seal, E Tomasella, K B Seroogy, T Hökfelt, G F Gebhart, P R Brumovsky
• Journal: Neuroscience (Neuroscience) Vol. 248 Pg. 95-111 (Sep 17 2013) ISSN: 1873-7544 [Electronic] United States
• PMID: 23727452 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Amino Acid Transport Systems, Acidic
• RNA, Messenger
• Slc17a6 protein, mouse
• Slc17a7 protein, mouse
• Vesicular Glutamate Transport Protein 1
• Vesicular Glutamate Transport Protein 2
• Vesicular Glutamate Transport Proteins
• vesicular glutamate transporter 3, mouse

Topics

• Amino Acid Transport Systems, Acidic (analysis, metabolism)
• Animals
• Axotomy
• Ganglia, Spinal (metabolism)
• Hindlimb
• Inflammation (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Neurons (metabolism)
• RNA, Messenger (metabolism)
• Sciatic Nerve (injuries)
• Spinal Cord (metabolism)
• Vesicular Glutamate Transport Protein 1 (analysis, metabolism)
• Vesicular Glutamate Transport Protein 2 (analysis, metabolism)
• Vesicular Glutamate Transport Proteins (analysis, metabolism)