Abstract | OBJECTIVE: The aim of the present study was to investigate the effects of plant extracts on cancer apoptotic induction. MATERIAL AND METHOD: Human epidermoid carcinoma A431 cell line, obtained from the American Type Culture Collection (ATCC, Manassas, VA), was maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 degrees C, 5% carbon dioxide (CO2). Plant extract solutions were obtained from S & J international enterprises public company limited. These plant extracts include 50% hydroglycol extracts from Etlingera elatior (Jack) R.M.Smith (torch ginger; EE), Rosa damascene (damask rose; DR) and Rafflesia kerrii Meijer (bua phut; RM). The cell viability, time and dose dependency were determined by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. A431 cells were treated with the plant extracts and stained with Hoechst 33342 fluorescent staining dye. RESULTS: Cell viability was demonstrated by the inhibitory concentration 50% (IC50). The anti-proliferative effects were shown to be dependent on time and dose. Typical characteristics of apoptosis which are cell morphological changes and chromatin condensation were clearly observed. CONCLUSION: The plant extracts was shown to be effective for anti-proliferation and induction of apoptosis cell death in skin cancer cells. Therefore, mechanisms underlying the cell death and its potential use for treatment of skin cancer will be further studied.
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Authors | Walairat Thuncharoen, Malin Chulasiri, Sirinun Nilwarangkoon, Yukio Nakamura, Ramida Watanapokasin |
Journal | Journal of the Medical Association of Thailand = Chotmaihet thangphaet
(J Med Assoc Thai)
Vol. 96 Suppl 1
Pg. S60-4
(Jan 2013)
ISSN: 0125-2208 [Print] Thailand |
PMID | 23724457
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Flowers
(chemistry)
- Humans
- Plant Extracts
(pharmacology)
- Rosa
(chemistry)
- Skin Neoplasms
(drug therapy)
- Staining and Labeling
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