Effects of botulinum toxin type A on expression of genes in keloid fibroblasts.

Abstract

BACKGROUND:

Invasive growth of fibroblast cells, which is regulated by multiple biological factors, is the key event in the pathophysiology of keloid scars. Recent studies have suggested that botulinum toxin type A (BoNT-A) could inhibit invasive growth of keloids. However, the molecular mechanisms are unknown.

OBJECTIVE:

The authors explore the effect of BoNT-A on the expression of genes relevant to invasive growth in keloid fibroblasts.

METHODS:

With 112 genes that were relevant to invasive growth, the authors utilized microarray analysis to study messenger RNA expression profiles in keloid fibroblasts treated with BoNT-A. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to confirm the microarray results.

RESULTS:

Analyses from microarray and qRT-PCR revealed that the S100A4 gene was upregulated and that the TGF-β1, VEGF, MMP-1, and PDGFA genes were downregulated in fibroblasts treated with BoNT-A.

CONCLUSIONS:

The BoNT-A altered expression levels of S100A4, TGF-β1, VEGF, MMP-1, and PDGFA genes in keloid fibroblasts provide a useful clue for exploring the function of BoNT-A and finding a novel treatment for keloid scarring.

• Authors: Wang Xiaoxue, Chen Xi, Xiao Zhibo
• Journal: Aesthetic surgery journal (Aesthet Surg J) Vol. 34 Issue 1 Pg. 154-9 (Jan 01 2014) ISSN: 1527-330X [Electronic] England
• PMID: 23709452 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dermatologic Agents
• Platelet-Derived Growth Factor
• RNA, Messenger
• S100 Calcium-Binding Protein A4
• S100 Proteins
• TGFB1 protein, human
• Transforming Growth Factor beta1
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• platelet-derived growth factor A
• S100A4 protein, human
• Botulinum Toxins, Type A
• incobotulinumtoxinA
• MMP1 protein, human
• Matrix Metalloproteinase 1

Topics

• Botulinum Toxins, Type A (pharmacology)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Dermatologic Agents (pharmacology)
• Fibroblasts (drug effects, metabolism, pathology)
• Gene Expression Profiling (methods)
• Gene Expression Regulation (drug effects)
• Humans
• Keloid (drug therapy, genetics, metabolism, pathology)
• Matrix Metalloproteinase 1 (genetics)
• Oligonucleotide Array Sequence Analysis
• Platelet-Derived Growth Factor (genetics)
• RNA, Messenger (metabolism)
• Real-Time Polymerase Chain Reaction
• S100 Calcium-Binding Protein A4
• S100 Proteins (genetics)
• Skin (drug effects, metabolism, pathology)
• Time Factors
• Transforming Growth Factor beta1 (genetics)
• Vascular Endothelial Growth Factor A (genetics)