Identification of human bocaviruses in the cerebrospinal fluid of children hospitalized with encephalitis in China.

Abstract

BACKGROUND:

Encephalitis is a major cause of death and disability in adults and children; different members in the family Parvoviridae are known to be associated with encephalitis to some extent.

OBJECTIVES:

To determine the presence of human bocaviruses (HBoVs) and corresponding HBoV-specific immunoglobulins (Igs) in cerebrospinal fluid from children with suspected viral encephalitis.

STUDY DESIGN:

Employing real-time PCR and nested touchdown PCR, 67 cerebrospinal fluid (CSF) samples from children with suspected viral encephalitis were screened for HBoV and routine encephalitis-causing viruses. Using ELISA, Western blot and IFA, HBoV-specific Ig were determined in the samples.

RESULTS:

Nine samples (134%) were HBoV1 DNA-positive, while one sample (15%) was HBoV2 DNA-positive. HBoV-specific IgM and IgG antibodies were detected in the CSF samples from three children; two samples were HBoV1 DNA-positive and one sample was negative. One death was recorded; CSF from this child was the only HBoV-IgM-positive CSF samples among the 67 CSF tested.

CONCLUSION:

We screened CSF samples obtained from children with encephalitis for the presence of HBoV1 and HBoV2 and specific IgM- or IgG-responses. Detection of viral DNA and/or immunological response to HBoV1/HBoV2 highlights the significance of these viruses as causes of encephalitis in children. Further studies are needed to examine the role of HBoV infection in children encephalitis.

• Authors: Jie-mei Yu, Qian-qian Chen, Ye-xia Hao, Tian Yu, Sai-zhen Zeng, Xiao-bing Wu, Bing Zhang, Zhao-jun Duan
• Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (J Clin Virol) Vol. 57 Issue 4 Pg. 374-7 (Aug 2013) ISSN: 1873-5967 [Electronic] Netherlands
• PMID: 23707415 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• DNA, Viral
• Immunoglobulin G
• Immunoglobulin M

Topics

• Child
• Child, Hospitalized
• Child, Preschool
• China
• DNA, Viral (genetics, immunology)
• Encephalitis, Viral (cerebrospinal fluid, immunology, virology)
• Female
• Human bocavirus (genetics, immunology)
• Humans
• Immunoglobulin G (immunology)
• Immunoglobulin M (immunology)
• Infant
• Male