Abstract | BACKGROUND:
Encephalitis is a major cause of death and disability in adults and children; different members in the family Parvoviridae are known to be associated with encephalitis to some extent. OBJECTIVES: To determine the presence of human bocaviruses (HBoVs) and corresponding HBoV-specific immunoglobulins (Igs) in cerebrospinal fluid from children with suspected viral encephalitis. STUDY DESIGN: Employing real-time PCR and nested touchdown PCR, 67 cerebrospinal fluid (CSF) samples from children with suspected viral encephalitis were screened for HBoV and routine encephalitis-causing viruses. Using ELISA, Western blot and IFA, HBoV-specific Ig were determined in the samples. RESULTS: Nine samples (134%) were HBoV1 DNA-positive, while one sample (15%) was HBoV2 DNA-positive. HBoV-specific IgM and IgG antibodies were detected in the CSF samples from three children; two samples were HBoV1 DNA-positive and one sample was negative. One death was recorded; CSF from this child was the only HBoV- IgM-positive CSF samples among the 67 CSF tested. CONCLUSION: We screened CSF samples obtained from children with encephalitis for the presence of HBoV1 and HBoV2 and specific IgM- or IgG-responses. Detection of viral DNA and/or immunological response to HBoV1/HBoV2 highlights the significance of these viruses as causes of encephalitis in children. Further studies are needed to examine the role of HBoV infection in children encephalitis.
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Authors | Jie-mei Yu, Qian-qian Chen, Ye-xia Hao, Tian Yu, Sai-zhen Zeng, Xiao-bing Wu, Bing Zhang, Zhao-jun Duan |
Journal | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
(J Clin Virol)
Vol. 57
Issue 4
Pg. 374-7
(Aug 2013)
ISSN: 1873-5967 [Electronic] Netherlands |
PMID | 23707415
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier B.V. All rights reserved. |
Chemical References |
- DNA, Viral
- Immunoglobulin G
- Immunoglobulin M
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Topics |
- Child
- Child, Hospitalized
- Child, Preschool
- China
- DNA, Viral
(genetics, immunology)
- Encephalitis, Viral
(cerebrospinal fluid, immunology, virology)
- Female
- Human bocavirus
(genetics, immunology)
- Humans
- Immunoglobulin G
(immunology)
- Immunoglobulin M
(immunology)
- Infant
- Male
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