Thrombin split products (prothrombin fragment 1 + 2) in urine in patients with suspected deep vein thrombosis admitted for radiological verification.

Abstract

INTRODUCTION:

The appearance of prothrombin fragment 1 + 2 (F1 + 2) in urine has been associated with postoperative hypercoagulability and thromboembolism. We wanted to assess if F1 + 2 was released in urine (uF1 + 2) in patients with procoagulant disorders, and if higher levels were found in patients with radiological verified deep vein thrombosis (DVT).

MATERIALS AND METHODS:

Consecutive patients were interviewed on comorbidities and medications. An unselected total cohort (n = 534) and a control cohort (n = 177) were analysed. A urine sample (10 ml) was collected and snap frozen before levels of uF1 + 2 were measured with an ELISA kit. Visualisation of DVT was done with compression ultrasound, supplied with venography when feasible. All patients were followed up for 3-6 months.

RESULTS:

DVT was diagnosed in 108/534 patients. Statistical significant higher uF1 + 2 levels were found in patients with DVT (p < 0.001), in DVT positive patients with ongoing malignancy (p = 0.034) and in pregnant women compared to the control cohort (p < 0.001). Non-significant increased urine concentrations were found in DVT positive vs. DVT negative patients with infections and traumas.

CONCLUSIONS:

Levels of uF1 + 2 was associated with DVT both in the total cohort and in the control cohort as well as in most patients with coexisting conditions.

• Authors: Fredrik Wexels, Anniken Haslund, Ola E Dahl, Are H Pripp, Tor E Gudmundsen, Ferencz Laszlo, Ingebjørg Seljeflot, Lars C Borris, Michael R Lassen
• Journal: Thrombosis research (Thromb Res) Vol. 131 Issue 6 Pg. 560-3 (Jun 2013) ISSN: 1879-2472 [Electronic] United States
• PMID: 23680105 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Peptide Fragments
• prothrombin fragment 1.2
• Prothrombin

Topics

• Adult
• Aged
• Aged, 80 and over
• Cohort Studies
• Female
• Follow-Up Studies
• Humans
• Male
• Middle Aged
• Peptide Fragments (urine)
• Pregnancy
• Prothrombin (urine)
• Ultrasonography
• Veins (diagnostic imaging)
• Venous Thrombosis (diagnosis, diagnostic imaging, urine)
• Young Adult