MicroRNA-17-92 cluster regulates osteoblast proliferation and differentiation.

Abstract

MicroRNAs (miRNAs) have been identified to play important functions during osteoblast proliferation, differentiation, and apoptosis. The miR-17~92 cluster is highly conserved in all vertebrates. Loss-of-function of the miR-17-92 cluster results in smaller embryos and immediate postnatal death of all animals. Germline hemizygous deletions of MIR17HG are accounted for microcephaly, short stature, and digital abnormalities in a few cases of Feingold syndrome. These reports indicate that miR-17~92 may play important function in skeletal development and mature. To determine the functional roles of miR-17~92 in bone metabolism as well as osteoblast proliferation and differentiation. Murine embryonic stem cells D3 and osteoprogenitor cell line MC3T3-E1 were induced to differentiate into osteoblasts; the expression of miR-17-92 was assayed by quantitative real-time RT-PCR. The skeletal phenotypes were assayed in mice heterozygous for miR-17~92 (miR-17~92 (+/Δ) ). To determine the possibly direct function of miR-17~92 in bone cells, osteoblasts from miR-17~92 (+/Δ) mice were investigated by ex vivo cell culture. miR-17, miR-92a, and miR-20a within miR-17-92 cluster were expressed at high level in bone tissue and osteoblasts. The expression of miR-17-92 was down-regulated along with osteoblast differentiation, the lowest level was found in mature osteoblasts. Compared to wildtype controls, miR-17-92 (+/Δ) mice showed significantly lower trabecular and cortical bone mineral density, bone volume and trabecular number at 10 weeks old. mRNA expression of Runx2 and type I collagen was significantly lower in bone from miR-17-92 (+/Δ) mice. Osteoblasts from miR-17-92 (+/Δ) mice showed lower proliferation rate, ALP activity and less calcification. Our research suggests that the miR-17-92 cluster critically regulates bone metabolism, and this regulation is mostly through its function in osteoblasts.

• Authors: Mingliang Zhou, Junrong Ma, Shiju Chen, Xiang Chen, Xijie Yu
• Journal: Endocrine (Endocrine) Vol. 45 Issue 2 Pg. 302-10 (Mar 2014) ISSN: 1559-0100 [Electronic] United States
• PMID: 23673870 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN17-92 microRNA, mouse
• MicroRNAs

Topics

• Animals
• Bone and Bones (cytology, metabolism)
• Cell Differentiation (genetics, physiology)
• Cell Line
• Cell Proliferation
• Down-Regulation (physiology)
• Embryonic Stem Cells (cytology, physiology)
• Genotype
• Homeostasis (genetics, physiology)
• In Vitro Techniques
• Mice
• MicroRNAs (genetics, physiology)
• Models, Animal
• Osteoblasts (cytology, physiology)
• Stem Cells (cytology, physiology)