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[Overexpression of eIF4E gene in acute myeloid leukemia and its relation with disease progression].

Abstract
The aim of this study was to detect the expression level of eIF4E gene in patients with non-treated, remission and non-remission/relapse acute myeloid leukemia (AML), and other non-malignant haematologic diseases so as to analyze and reveal the relationship of eIF4E gene expression with AML progression. SYBR Green I RT-PCR was used to assay the expression level of eIF4E mRNA extracted from bone marrow mononuclear cells in 30 patients with AML (6 in M2, 5 in M3, 8 in M4, 10 in M5, 1 in M6) and 20 patients with non-malignant hematologic diseases. The β2-microglubin(β2M) was used as internal reference and the formula 2(-ΔCt)×100% was applied to calculate the expression level of eIF4E gene. The results showed that the eIF4E expression level (7.098 ± 5.544)% in patients with non-treated and non-remitted/relapsed AML was significantly higher than that in patients with remission (0.964 ± 0.312)% (P < 0.01) and non-malignant hematologic diseases (0.248 ± 0.163)% (P < 0.01). There was no difference between latter two group patients, even though the expression level of eIF4E gene in patients with M4 and M5 was higher. As compared with non-malignant hematologic diseases, the expression level of eIF4E gene of patients with remission patients showed no significant difference. It is concluded that the over-expression of eIF4E gene has been found in patients with AML, and its level obviously decreases along with remission of disease, thus the eIF4E gene may be a surveillance parameter for disease progression.
AuthorsYin-Di Jiang, Yu-Hong Lu, Shao-Hua Chen, Kang-Er Zhu, Xue-Li Zhang, Zhi Yu, Jun Zhong, Tao Zhang, Geng-Xin Luo, Jie Chen, Huan-Yu Pan, Yan Li, Lian-An Qin, Yang-Qiu Li
JournalZhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 21 Issue 2 Pg. 296-9 (Apr 2013) ISSN: 1009-2137 [Print] China
PMID23628019 (Publication Type: Controlled Clinical Trial, English Abstract, Journal Article)
Chemical References
  • Eukaryotic Initiation Factor-4E
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Disease Progression
  • Eukaryotic Initiation Factor-4E (genetics)
  • Female
  • Gene Expression
  • Humans
  • Leukemia, Myeloid, Acute (genetics, pathology)
  • Male
  • Middle Aged
  • Real-Time Polymerase Chain Reaction
  • Young Adult

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