Abstract |
The effects of pyrroloquinoline quinine (PQQ) on RANKL-induced osteoclast differentiation and on wear particle-induced osteolysis were examined in this study. PQQ inhibited RANKL-mediated osteoclast differentiation in bone marrow macrophages (BMMs) in a dose-dependent manner without any evidence of cytotoxicity. The mRNA expression of c-Fos, NFATc1, and TRAP in RANKL-treated BMMs was inhibited by PQQ treatment. Moreover, RANKL-induced c-Fos and NFATc1 protein expression was suppressed by PQQ. PQQ additionally inhibited the bone resorptive activity of differentiated osteoclasts. Further a UHMWPE-induced murine calvaria erosion model study was performed to assess the effects of PQQ on wear particle-induced osteolysis in vivo. Mice treated with PQQ demonstrated marked attenuation of bone erosion based on Micro-CT and histologic analysis of calvaria. These results collectively suggested that PQQ demonstrated inhibitory effects on osteoclast differentiation in vitro and may suppress wear particle-induced osteolysis in vivo, indicating that PQQ may therefore serve as a useful drug in the prevention of bone loss.
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Authors | Lingbo Kong, Chongfei Yang, Lifeng Yu, Wanli Smith, Shu Zhu, Jinyu Zhu, Qingsheng Zhu |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 4
Pg. e61013
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23613773
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Isoenzymes
- NFATC Transcription Factors
- Polyethylenes
- Proto-Oncogene Proteins c-fos
- Pyrroles
- Quinolines
- RANK Ligand
- RNA, Messenger
- pyrroloquinoline
- ultra-high molecular weight polyethylene
- Quinine
- Acid Phosphatase
- Acp5 protein, mouse
- Tartrate-Resistant Acid Phosphatase
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Topics |
- Acid Phosphatase
(genetics, metabolism)
- Animals
- Bone Marrow Cells
(drug effects, metabolism)
- Cell Death
(drug effects)
- Cell Differentiation
(drug effects)
- Gene Expression Regulation
(drug effects)
- Isoenzymes
(genetics, metabolism)
- Mice
- Mice, Inbred C57BL
- NFATC Transcription Factors
(genetics, metabolism)
- Osteoclasts
(drug effects, metabolism, pathology)
- Osteogenesis
(drug effects)
- Osteolysis
(chemically induced, metabolism, pathology)
- Polyethylenes
(adverse effects)
- Proto-Oncogene Proteins c-fos
(genetics, metabolism)
- Pyrroles
(pharmacology)
- Quinine
(pharmacology)
- Quinolines
(pharmacology)
- RANK Ligand
(pharmacology)
- RNA, Messenger
(genetics, metabolism)
- Skull
(diagnostic imaging, pathology)
- Tartrate-Resistant Acid Phosphatase
- X-Ray Microtomography
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