[The expression and function of anti-apoptotic Bfl-1 in prostate cancer].

Abstract

OBJECTIVE:

To determine the expression of Bcl2 related protein A1(Bfl-1) mRNA in prostate cancer cell lines and tissues, and to explore the functions of Bfl-1 in prostate adenocarcinoma.

METHODS:

RT-PCR, real-time quantitative PCR (Q-PCR)and in situ hybridization (ISH) were used to detect the expression of Bfl-1 mRNA in prostate cancer cell lines, tissues and benign prostate hyperplasia (BPH) tissue samples. The relationship between Bfl-1 expression and clinicopathological parameters was analyzed. Antisense oligonucleotides (ASONs) were used to interfere the expression of Bfl-1 and its effects on prostate cancer cells. MTT was used to detect the survival, morphologic changes of prostate cancer cells was observed by inverted microscope.

RESULTS:

Bfl-1 mRNA, detected by RT-PCR, Q-PCR and ISH, was overexpressed in the androgen-independent prostate cancer cell lines PC-3 and DU145, but not detectable in the androgen-dependent prostate cancer cell line LNCaP and BPH tissue samples (P < 0.05). Significantly higher Bfl-1 mRNA levels were observed in higher stage and metastatic prostate cancer cases than those without metastasis or of low stage. ASONs targeting Bfl-1 significantly inhibited androgen-independent prostate cancer cell growth (P < 0.05), cell was rounding off or fragmentation.

CONCLUSION:

Bfl-1 is involved in maintaining the hormone-independent prostate cancer cell growth. Bfl-1 may become a new therapeutic target in advanced prostate cancer.

• Authors: Xing-Lan Li, Xue-Qin Chen, Ling Nie, Miao Xu, Qiu-Yao Li, Wei-Wei Shang, Ni Chen, Rui Huang, Hao Zeng, Qiao Zhou
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 44 Issue 1 Pg. 21-6 (Jan 2013) ISSN: 1672-173X [Print] China
• PMID: 23600202 (Publication Type: Journal Article)

Chemical References

• BCL2-related protein A1
• Minor Histocompatibility Antigens
• Oligonucleotides, Antisense
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger

Topics

• Apoptosis
• Cell Line
• Cell Transformation, Neoplastic
• Humans
• In Situ Hybridization
• Male
• Minor Histocompatibility Antigens
• Oligonucleotides, Antisense
• Prostatic Hyperplasia (metabolism)
• Prostatic Neoplasms (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• RNA, Messenger