Intravenous transfer of apoptotic cell-treated dendritic cells leads to immune tolerance by blocking Th17 cell activity.

Abstract

Apoptotic cell-induced tolerogenic dendritic cells (DCs) play an important role in induction of peripheral tolerance in vivo; however, the mechanisms of immune tolerance induced by these DCs are poorly understood. Here we show that treatment of apoptotic cells modulates expression of inflammation- and tolerance-associated molecules including Gr-1, B220, CD205 and galectin-1 on bone marrow-derived DCs. In addition, apoptotic cell-treated DCs suppress secretion of cytokines produced by Th17 cells. Our data also demonstrate that i.v. transfer of apoptotic cell-treated DCs blocks EAE development and down-regulates production of inflammatory cytokines such as IL-17A and IL-17F in CD4+ T cells. These results suggest that apoptotic cell-treated DCs may inhibit activity of Th17 cells via down-regulation of inflammatory cytokine production, thereby affecting EAE development in vivo. Our results reveal a potential mechanism of immune tolerance mediated by apoptotic cell-treated DCs and the possible use of apoptotic cell-treated DCs to treat autoimmune diseases such as MS/EAE.

• Authors: Fang Zhou, Elisabetta Lauretti, Antonio di Meco, Bogoljub Ciric, Patricia Gonnella, Guang-Xian Zhang, Abdolmohamad Rostami
• Journal: Immunobiology (Immunobiology) Vol. 218 Issue 8 Pg. 1069-76 (Aug 2013) ISSN: 1878-3279 [Electronic] Netherlands
• PMID: 23587571 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier GmbH. All rights reserved.

Chemical References

• Antigens, CD
• Cytokines
• DEC-205 receptor
• Galectin 1
• Gr-1 protein, mouse
• Lectins, C-Type
• Minor Histocompatibility Antigens
• Myelin-Oligodendrocyte Glycoprotein
• Peptide Fragments
• Receptors, Cell Surface
• Receptors, Chemokine
• myelin oligodendrocyte glycoprotein (35-55)
• Leukocyte Common Antigens

Topics

• Animals
• Antigens, CD (biosynthesis)
• Apoptosis (immunology)
• Bone Marrow Cells (immunology)
• Cell Differentiation (immunology)
• Cells, Cultured
• Cytokines (biosynthesis)
• Dendritic Cells (immunology, metabolism)
• Encephalomyelitis, Autoimmune, Experimental (immunology, therapy)
• Female
• Galectin 1 (biosynthesis)
• Immune Tolerance (immunology)
• Immunotherapy
• Lectins, C-Type (biosynthesis)
• Leukocyte Common Antigens (biosynthesis)
• Mice
• Mice, Inbred C57BL
• Minor Histocompatibility Antigens
• Myelin-Oligodendrocyte Glycoprotein (administration & dosage, immunology)
• Peptide Fragments (administration & dosage, immunology)
• Receptors, Cell Surface (biosynthesis)
• Receptors, Chemokine (biosynthesis)
• Th17 Cells (immunology, metabolism)