HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Detection of cytokeratin-20-positive cells in preoperative and postoperative blood samples from colorectal cancer patients by real-time RT-PCR.

AbstractBACKGROUND:
Detection of circulating cancer cells by reverse transcription polymerase chain reaction (RT-PCR) has been studied as a prognostic marker in patients with colorectal cancer (CRC) but so far with conflicting results regarding specificity and prognostic value. In this study cytokeratin-20 (CK20) was evaluated by real-time RT-PCR as a marker for circulating CRC cell detection and the influence of surgical tumor resection on the presence of circulating CRC cells was analyzed.
METHODS:
RNA was isolated from the mononuclear cell fraction of blood samples taken from 95 CRC patients before and after tumor resection and from 23 healthy volunteers and assayed by real-time RT-PCR for CK20 expression.
RESULTS:
Among 23 healthy volunteers one was positive for CK20. Among 95 CRC patients, 25 were positive for CK20 before and 23 after surgery. Sixteen patients positive before surgery became negative after surgery, while 14 patients negative before surgery became positive after surgery. An increase in the proportion of CK20-positive samples with increasing stage of disease was observed for preoperative but not postoperative blood samples.
CONCLUSIONS:
Its association with clinical stage indicates that CK20 might have prognostic value as a marker for detection of circulating CRC cells. Surgical tumor resection can both reduce and induce the presence of circulating CRC cells.
AuthorsIvan Šamija, Josip Lukač, Mirjana Končar Mubrin, Iva Kirac, Dujo Kovačević, Zvonko Kusić
JournalThe International journal of biological markers (Int J Biol Markers) Vol. 28 Issue 2 Pg. 174-81 (Jun 28 2013) ISSN: 1724-6008 [Electronic] United States
PMID23558939 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers, Tumor
  • Keratin-20
  • RNA, Messenger
Topics
  • Adult
  • Aged
  • Biomarkers, Tumor (blood)
  • Colorectal Neoplasms (blood, diagnosis, surgery)
  • Female
  • Healthy Volunteers
  • Humans
  • Keratin-20 (blood)
  • Male
  • Middle Aged
  • Neoplastic Cells, Circulating
  • Postoperative Period
  • Preoperative Period
  • Prognosis
  • RNA, Messenger (blood, isolation & purification)
  • Reverse Transcriptase Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: