Abstract |
We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID50) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β- galactosidase, which was used to determine TCID50 levels. Validation of TCID50 values was performed by immunofluorescence assay of HHV-8 infection of immature dendritic cells at various TCID50 doses.
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Authors | Sagar V Nadgir, Heather R Hensler, Emilee R Knowlton, Charles R Rinaldo, Giovanna Rappocciolo, Frank J Jenkins |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 51
Issue 6
Pg. 1931-4
(Jun 2013)
ISSN: 1098-660X [Electronic] United States |
PMID | 23554189
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Validation Study)
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Chemical References |
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Topics |
- Cell Line
- Dendritic Cells
(virology)
- Genes, Reporter
- Herpesvirus 8, Human
(isolation & purification, pathogenicity, physiology)
- Humans
- Viral Load
(methods)
- beta-Galactosidase
(analysis)
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