Systemically administered liposome-encapsulated Ad-PEDF potentiates the anti-cancer effects in mouse lung metastasis melanoma.

Abstract

BACKGROUND:

The use of adenoviral vector for gene therapy is still an important strategy for advanced cancers, however, the lack of the requisite coxsackie-adenovirus receptor in cancer cells and host immune response to adenovirus limit the application of adenoviral vector in vivo.

METHOD:

We designed the antiangiogenic gene therapy with recombinant PEDF adenovirus (Ad-PEDF) encapsulated in cationic liposome (Ad-PEDF/Liposome), and investigated the anti-tumor efficacy of Ad-PEDF/Liposome complex on inhibition of tumor metastasis.

RESULTS:

We found that systemic administration of Ad-PEDF/liposome was well tolerated and resulted in marked suppression of tumor growth, and was more potent than uncoated Ad-PEDF to induce apoptosis in B16-F10 melanoma cells and inhibit murine pulmonary metastases in vivo. After Ad-luciferase was encapsulated with liposome, its distribution decreased in liver and increased in lung. The anti-Ad IgG level of Ad-PEDF/Liposome was significantly lower than Ad-PEDF used alone.

CONCLUSION:

The present findings provide evidences of systematic administration of cationic liposome-encapsulated Ad-PEDF in pulmonary metastatic melanoma mice model, and show an encouraging therapeutic effect for further exploration and application of more complexes based on liposome-encapsulated adenovirus for more cancers.

• Authors: Hua-shan Shi, Li-ping Yang, Wei Wei, Xiao-qing Su, Xiao-peng Li, Meng Li, Shun-tao Luo, Hai-long Zhang, Lian Lu, Yong-qiu Mao, Bing Kan, Li Yang
• Journal: Journal of translational medicine (J Transl Med) Vol. 11 Pg. 86 (Apr 03 2013) ISSN: 1479-5876 [Electronic] England
• PMID: 23552524 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cations
• Eye Proteins
• Immunoglobulin G
• Liposomes
• Nerve Growth Factors
• Serpins
• pigment epithelium-derived factor

Topics

• Adenoviridae (genetics)
• Animals
• Cations
• Eye Proteins (genetics)
• Female
• Genetic Therapy (methods)
• Genetic Vectors
• Immunoglobulin G (chemistry)
• Liposomes (chemistry, metabolism)
• Lung Neoplasms (pathology, therapy)
• Melanoma (pathology, therapy)
• Melanoma, Experimental
• Mice
• Mice, Inbred C57BL
• Neoplasm Metastasis
• Nerve Growth Factors (genetics)
• Serpins (genetics)