Osthole ameliorates renal ischemia-reperfusion injury by inhibiting inflammatory response.

Abstract

INTRODUCTION:

Renal ischemia-reperfusion (I/R) injury is a primary cause of acute renal failure that results in high mortality. This study aimed to investigate the effect of osthole, a natural coumarin derivative, on renal I/R injury in a rat model.

MATERIALS AND METHODS:

Rats were randomly allocated to the sham operation + vehicle, I/R + vehicle, and I/R + osthole groups. Renal I/R injury was induced by clamping the left renal artery for 45 min followed by 12 h of reperfusion and a contralateral nephrectomy. Osthole (40 mg/kg) was intraperitoneally injected 30 min before inducing I/R. Renal function and histological damage were determined subsequently. Myeloperoxidase activity, monocyte/macrophage infiltration, as well as tumor necrosis factor-α, IL-1β, and activated p38 mitogen-activated protein kinase expression in kidneys were also assessed.

RESULTS:

Osthole treatment significantly ameliorated I/R-induced renal functional and morphological injuries. Moreover, osthole treatment attenuated myeloperoxidase activity, monocyte/macrophage infiltration, and tumor necrosis factor-α, IL-1β, and activated p38 mitogen-activated protein kinase expression in kidneys.

CONCLUSIONS:

Osthole treatment ameliorates renal I/R injury by inhibiting inflammatory responses in kidneys. Thus, osthole may represent a novel practical strategy to prevent renal I/R injury.

• Authors: Yi Zheng, Min Lu, Lulin Ma, Shudong Zhang, Min Qiu, Xin Ma
• Journal: Urologia internationalis (Urol Int) Vol. 91 Issue 3 Pg. 350-6 ( 2013) ISSN: 1423-0399 [Electronic] Switzerland
• PMID: 23548945 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 S. Karger AG, Basel.

Chemical References

• Calcium Channel Blockers
• Coumarins
• Interleukin-1beta
• Tumor Necrosis Factor-alpha
• Peroxidase
• p38 Mitogen-Activated Protein Kinases
• osthol

Topics

• Acute Kidney Injury (prevention & control)
• Animals
• Calcium Channel Blockers (therapeutic use)
• Coumarins (therapeutic use)
• Disease Models, Animal
• Gene Expression Regulation, Enzymologic
• Inflammation (prevention & control)
• Interleukin-1beta (metabolism)
• Kidney (drug effects, enzymology)
• Male
• Monocytes (cytology)
• Peroxidase (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (therapy)
• Tumor Necrosis Factor-alpha (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)