Abstract | BACKGROUND: AIM AND METHODS: To identify cytokine responses associated with pulmonary tuberculosis (TB), TB lymphadenitits and latent TB, we examined mycobacterial antigen-specific immune responses of PTB, TBL and LTB individuals. More specifically, we examined ESAT-6 and CFP-10 induced Type 1, Type 2 and Type 17 cytokine production and their regulation using multiplex ELISA. RESULTS: PTB individuals exhibited a significantly lower baseline as well as antigen-specific production of Type 1 (IFNγ, TNFα and IL-2); Type 2 (IL-4) and Type 17 (IL-17A and IL-17F) cytokines in comparison to both TBL and LTB individuals. TBL individuals exhibited significantly lower antigen-specific IFNγ responses alone in comparison to LTB individuals. Although, IL-10 levels were not significantly higher, neutralization of IL-10 during antigen stimulation resulted in significantly enhanced production of IFNγ, IL-4 and IL-17A in PTB individuals, indicating that IL-10 mediates (at least partially) the suppression of cytokine responses in PTB. CONCLUSION: Pulmonary TB is characterized by an IL-10 dependent antigen-specific suppression of Type 1, Type 2 and Type 17 cytokines, reflecting an important association of these cytokines in the pathogenesis of active TB.
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Authors | Nathella Pavan Kumar, Venugopal Gopinath, Rathinam Sridhar, Luke E Hanna, Vaithilingam V Banurekha, Mohideen S Jawahar, Thomas B Nutman, Subash Babu |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 3
Pg. e59572
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23544075
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
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Chemical References |
- Antigens, Bacterial
- Cytokines
- IL10 protein, human
- Interleukin-10
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Topics |
- Adult
- Aged
- Antigens, Bacterial
(immunology)
- Cytokines
(biosynthesis)
- Female
- Humans
- Immunomodulation
- Interleukin-10
(metabolism)
- Male
- Middle Aged
- Neutralization Tests
- Tuberculosis, Pulmonary
(immunology, microbiology, pathology)
- Young Adult
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