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Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism.

Abstract
Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and (31)P NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.
AuthorsMarina Stanić, Joanna Zakrzewska, Mirzeta Hadžibrahimović, Milan Zižić, Zoran Marković, Zeljko Vučinić, Miroslav Zivić
JournalResearch in microbiology (Res Microbiol) Vol. 164 Issue 7 Pg. 770-8 (Sep 2013) ISSN: 1769-7123 [Electronic] France
PMID23542427 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Chemical References
  • Fungal Proteins
  • Mitochondrial Proteins
  • Phosphates
  • Plant Proteins
  • Adenosine Triphosphate
  • Oxidoreductases
  • alternative oxidase
  • Oxygen
Topics
  • Adenosine Triphosphate (metabolism)
  • Energy Metabolism
  • Fungal Proteins (metabolism)
  • Mitochondrial Proteins (metabolism)
  • Oxidoreductases (metabolism)
  • Oxygen (metabolism)
  • Phosphates (metabolism)
  • Phycomyces (enzymology, metabolism)
  • Plant Proteins (metabolism)

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