Abstract | OBJECTIVE: METHOD AND MATERIALS: THP-1 monocytes were cultured and differentiated into MØ. DHA was added to MØ in a dose-dependent manner. MØ samples were added to the following groups: Group 1, ethanol alone as a solvent control; Group 2, 10 µg/ml of DHA (D1); Group 3, 20 µg/ml of DHA (D2); Group 4, 10 µg/ml of DHA + LPS (DL1); Group 5, 20 µg/ml of DHA + LPS (DL2); Group 6, LPS alone. Reverse transcriptase-PCR (RT-PCR) followed by ImageJ analysis was used to measure cytokine expression. RESULTS: The results show that IL-1ß and TNF-α levels for DL and DHA (basal) were significantly lower than the levels in LPS alone. IL-6 was increased in the DL groups. There was no significant change for IL-8. CONCLUSION: DHA at higher concentrations may selectively decrease proinflammatory cytokine production of IL-1ß and TNF-α. More studies are needed to verify the anti-inflammatory therapeutic action of agents like DHA omega-3 fatty acids.
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Authors | Poonam Solanki, Anita Aminoshariae, Ge Jin, Thomas A Montagnese, Andre Mickel |
Journal | Quintessence international (Berlin, Germany : 1985)
(Quintessence Int)
Vol. 44
Issue 6
Pg. 393
(Apr 24 2013)
ISSN: 1936-7163 [Electronic] Germany |
PMID | 23534044
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- CXCL8 protein, human
- IL6 protein, human
- Inflammation Mediators
- Interleukin-1beta
- Interleukin-6
- Interleukin-8
- Lipopolysaccharides
- Solvents
- Tumor Necrosis Factor-alpha
- Docosahexaenoic Acids
- Ethanol
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Topics |
- Cell Culture Techniques
- Cell Differentiation
(physiology)
- Cells, Cultured
- Docosahexaenoic Acids
(administration & dosage, pharmacology)
- Dose-Response Relationship, Drug
- Ethanol
(pharmacology)
- Humans
- Inflammation Mediators
(analysis)
- Interleukin-1beta
(immunology, metabolism)
- Interleukin-6
(immunology, metabolism)
- Interleukin-8
(immunology, metabolism)
- Lipopolysaccharides
(immunology, pharmacology)
- Macrophage Activation
(drug effects, immunology)
- Macrophages
(drug effects, immunology, metabolism)
- Monocytes
(physiology)
- Solvents
(pharmacology)
- Tumor Necrosis Factor-alpha
(drug effects, immunology)
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