Experimental research on treatment of injured facial nerves induced by hepatocyte growth factor mediated by ultrasound-targeted microbubble destruction.
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| Abstract |
The purpose of our study was to explore the regeneration effect of the ultrasound (US)-targeted microbubble destruction-mediated eukaryotic coexpression vector (pIRES2-enhanced green fluorescent protein [EGFP]/hepatocyte growth factor [HGF]) with EGFP and HGF gene system on facial nerve injury in rats. Forty rats were randomly divided into 4 groups after the models of facial nerve injury were established: A, phosphate-buffered saline (PBS) group; B, HGF and microbubble (HGF + MB) group; C, HGF and US (HGF + US) group; and D, HGF + US + microbubble (HGF + MB/US) group. Gene and protein levels of HGF were detected by quantitative real-time reverse transcriptase-polymerase chain reaction and Western blot, respectively. The expression of pEGFP in facial nerve trunks was examined by laser scanning confocal microscope; HGF gene and protein expression were significantly higher in D group compared with those of the other groups (P < 0.05). The expression of pEGFP was the strongest in D group (P < 0.05). These data indicate that US-targeted microbubble destruction effectively transfects the HGF gene into target tissues and has a significant effect on an injured facial nerve, thus providing a new strategy for gene therapy in facial nerve injury.
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| Authors | Ya-ning Hao, Wen-long Luo, Dan Wang, Zhi-gang Wang |
| Journal | The Journal of craniofacial surgery (J Craniofac Surg) Vol. 24 Issue 2 Pg. 421-4 (Mar 2013) ISSN: 1536-3732 [Electronic] United States |
| PMID | 23524706 (Publication Type: Journal Article) |
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