Reflux
laryngitis is a common clinic complication of nasogastric intubation (NSGI). Since there is no report concerning the effects of
low level laser therapy (
LLLT) on reflux
laryngitis, this study aimed to analyze the protective effect of single and combined
therapies with low level
laser at the doses of 2.1J and 2.1+1.2 J with a total irradiation time of 30s and 30+30 s, respectively, on a model of neurogenic reflux
laryngitis. NSGI was performed in Wistar rats, assigned into groups: NGI (no treatment), NLT17.5 (single
therapy), and NLT17.5/10.0 (combined
therapy, applied sequentially). Additional non-intubated and non-irradiated rats were use as controls (CTR).
Myeloperoxidase (MPO) activity was assessed by colorimetric method after the intubation period (on days 1, 3, 5, and 7), whereas
paraffin-embedded laryngeal specimens were used to carry out histopathological analysis of the inflammatory response, granulation tissue, and
collagen deposition 7 days after NSGI. Significant reduction in MPO activity (p<0.05) and in the severity of the inflammatory response (p<0.05), and improvement in the granulation tissue (p<0.05) was observed in NLT17.5/10.0 group. Mast cells count was significantly decreased in NGI and NLT17.5 groups (p<0.001), whereas no difference was observed between NLT17.5/10.0 and CTR groups (p>0.05). NLT17.5/10.0 group also showed better collagenization pattern, in comparison to NGI and NLT17.5 groups. This study suggests that the combined
therapy successfully modulated the inflammatory response and collagenization in experimental model of NSGI-induced neurogenic
laryngitis.