Abstract | BACKGROUND: METHODS: The adult healthy mice were divided into four groups, each consisting of ten subjects: mice in group Con-L(VT) and group Sev-L(VT) were ventilated with tidal volumes of 8 mL/kg for 4 hours, while those in group Con-H(VT) and group Sev-H(VT) were ventilated with tidal volumes of 16 mL/kg instead. Control mice (group Con-L(VT) and Con-H(VT)) were subjected to fresh air, while sevoflurane-treated mice (groups Sev- L(VT) and Sev-H(VT)) were subjected to air mixed with 1 vol% sevoflurane. After 4 hours of ventilation, the bronchoalveolar lavage (BAL) fluid was collected and analyzed for the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10. Lung homogenates were harvested to detect the expression of nuclear factor-kappa B (NF-κB) and heme oxygenase (HO)-1 mRNA by reverse transcription-polymerase chain reaction method. Lung damage was evaluated using the modified Ventilator-Induced Lung Injury histological scoring system. RESULTS: Compared to group Con-L(VT), the levels of TNF-α, IL-1β, IL-6, and IL-10 in BAL fluid, mRNA expressions of NF-κB and HO-1 in lung tissue, and lung injury scores were significantly increased in group Con-H(VT); compared to group Con-H(VT), group Sev-H(VT) BAL samples showed decreased levels of TNF-α, IL-1β, and IL-6; they also showed increased levels of IL-10, the downregulation of NF-κB mRNA, and HO-1 mRNA upregulation; the lung injury scores were significantly lower in group Sev-H(VT) than group Con-H(VT). CONCLUSION:
Mechanical ventilation with high tidal volume might lead to lung injury, which could be significantly, but not completely, attenuated by sevoflurane inhalation by inhibiting the NF-κB-mediated proinflammatory cytokine generation and upregulating HO-1 expression.
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Authors | Xiang-qing Xiong, Li-na Lin, Liang-rong Wang, Li-da Jin |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 6
Pg. 1075-81
( 2013)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 23515704
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Cytokines
- Membrane Proteins
- Methyl Ethers
- RNA, Messenger
- Sevoflurane
- Heme Oxygenase-1
- Hmox1 protein, mouse
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Topics |
- Analysis of Variance
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Bronchoalveolar Lavage Fluid
(chemistry)
- Cytokines
(analysis, metabolism)
- Heme Oxygenase-1
(genetics, metabolism)
- Hemodynamics
- Lung
(chemistry, metabolism, pathology)
- Male
- Membrane Proteins
(genetics, metabolism)
- Methyl Ethers
(pharmacology)
- Mice
- Mice, Inbred C57BL
- Pneumonia
(drug therapy, genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Sevoflurane
- Statistics, Nonparametric
- Up-Regulation
(drug effects)
- Ventilator-Induced Lung Injury
(drug therapy, genetics, metabolism)
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