A diet containing soybean oil heated for three hours increases adipose tissue weight but decreases body weight in C57BL/6 J mice.

Abstract

BACKGROUND:

Our previous work showed that dietary oxidized linoleic acid given, as a single fatty acid, to LDL receptor knockout mice decreased weight gain as compared to control mice. Other studies have also reported that animals fed oils heated for 24 h or greater showed reduced weight gain. These observations, while important, have limited significance since fried foods in the typical human diet do not contain the extreme levels of oxidized lipids used in these studies. The main goal of this study was to investigate the effects of a diet containing soybean oil heated for 3 h on weight gain and fat pad mass in mice. Additionally, because PPARγ and UCP-1 mediate adipocyte differentiation and thermogenesis, respectively, the effect of this diet on these proteins was also examined.

FINDINGS:

Four to six week old male C57BL/6 J mice were randomly divided into three groups and given either a low fat diet with heated soybean oil (HSO) or unheated soybean oil (USO) or pair fed for 16 weeks. Weight and food intake were monitored and fat pads were harvested upon the study's termination. Mice consuming the HSO diet had significantly increased fat pad mass but gained less weight as compared to mice in the USO group despite a similar caloric intake and similar levels of PPARγ and UCP1.

CONCLUSION:

This is the first study to show that a diet containing soybean oil heated for a short time increases fat mass despite a decreased weight gain in C57BL/6 J mice. The subsequent metabolic consequences of this increased fat mass merits further investigation.

• Authors: Meera Penumetcha, Mary K Schneider, Holly A Cheek, Sonia Karabina
• Journal: Lipids in health and disease (Lipids Health Dis) Vol. 12 Pg. 26 (Mar 06 2013) ISSN: 1476-511X [Electronic] England
• PMID: 23510583 (Publication Type: Journal Article)

Chemical References

• Dietary Fats
• Ion Channels
• Mitochondrial Proteins
• PPAR gamma
• UCP1 protein, human
• Ucp1 protein, mouse
• Uncoupling Protein 1
• Soybean Oil

Topics

• Adipose Tissue (drug effects, metabolism)
• Animals
• Body Composition (drug effects)
• Body Weight (drug effects)
• Cooking
• Diet, Fat-Restricted
• Dietary Fats (administration & dosage)
• Eating (drug effects)
• Energy Intake (drug effects)
• Gene Expression (drug effects)
• Hot Temperature
• Humans
• Ion Channels (genetics, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Mitochondrial Proteins (genetics, metabolism)
• PPAR gamma (genetics, metabolism)
• Soybean Oil (chemistry, pharmacology)
• Uncoupling Protein 1
• Weight Gain (drug effects)