MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia.

Abstract

Matrix metallinoprotease-9 (MMP9) plays a key role in the pathogenesis of post-ischemic blood brain barrier (BBB) disruption and the formation of lesions after cerebral ischemia. In this study we investigate the effect of brain-specific miRNAs on MMP-9 protein level in the rat hippocampus following cerebral ischemia and its underlying mechanism. Cerebral ischemia significantly upregulated miR-21 and -224 in the hippocampus; however, expression of miR-122 and -338-3p was not significantly affected by ischemia. Silencing of miR-21, but not -224, reduced MMP9 protein level after cerebral ischemia. Downregulation of extracellular signal-regulated kinase (ERK) signaling using the ERK inhibitor U0126 and the calcium-channel blocker ketamine inhibited the upregulation of miR-21 expression and MMP9 protein level after cerebral ischemia. The study suggests that cerebral ischemia up-regulates expression level of miR-21, which is involved in ERK-stimulated upregulation of MMP9 following cerebral ischemia via a calcium-dependent mechanism.

• Authors: XiaHeng Deng, Yun Zhong, LiZe Gu, Wei Shen, Jun Guo
• Journal: Brain research bulletin (Brain Res Bull) Vol. 94 Pg. 56-62 (May 2013) ISSN: 1873-2747 [Electronic] United States
• PMID: 23473787 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• MicroRNAs
• mirn21 microRNA, rat
• Matrix Metalloproteinase 9
• Mmp9 protein, rat

Topics

• Animals
• Blotting, Western
• Brain Ischemia (metabolism)
• Hippocampus (metabolism)
• MAP Kinase Signaling System (physiology)
• Male
• Matrix Metalloproteinase 9 (biosynthesis)
• MicroRNAs (metabolism)
• Rats
• Rats, Sprague-Dawley
• Real-Time Polymerase Chain Reaction
• Up-Regulation