Importance of transcript levels of caspase-2 isoforms S and L for breast carcinoma progression.

Abstract

AIM:

A role of caspase-2 in chemotherapy-induced apoptosis has been suggested. Our study aimed to evaluate the prognostic and predictive importance of caspase-2 isoforms in breast cancer patients.

MATERIALS & METHODS:

Caspase-2L and -2S transcript levels were determined in paired tumor and non-malignant control tissues from 64 patients after neoadjuvant chemotherapy and 100 pretreatment patients (general set) by real-time PCR with absolute quantification.

RESULTS:

Low but statistically significant upregulation of caspase-2L in tumor versus control tissues was observed in both sets. Significant associations of the levels of caspase-2L, -2S or S/L ratio with clinical prognostic factors were observed. However, none of these associations were confirmed in both sets. Levels of caspase-2 isoforms or the S/L ratio did not significantly associate with progression-free survival in the general set or with chemotherapy response in the neoadjuvant set.

CONCLUSION:

Our results suggest that the role of caspase-2 isoforms in the progression of breast cancer may considerably differ between pre- and post-chemotherapy patients.

• Authors: Veronika Brynychová, Viktor Hlaváč, Marie Ehrlichová, Radka Václavíková, Václav Pecha, Markéta Trnková, Martin Wald, Marcela Mrhalová, Kateřina Kubáčková, Tomáš Pikus, Roman Kodet, Jan Kovář, Pavel Souček
• Journal: Future oncology (London, England) (Future Oncol) Vol. 9 Issue 3 Pg. 427-38 (Mar 2013) ISSN: 1744-8301 [Electronic] England
• PMID: 23469978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoenzymes
• RNA, Messenger
• CASP2 protein, human
• Caspase 2
• Cysteine Endopeptidases

Topics

• Adult
• Aged
• Breast Neoplasms (enzymology, mortality)
• Carcinoma, Ductal, Breast (enzymology, mortality)
• Caspase 2 (genetics, metabolism)
• Chemotherapy, Adjuvant
• Cysteine Endopeptidases (genetics, metabolism)
• Female
• Gene Frequency
• Humans
• Isoenzymes (genetics, metabolism)
• Kaplan-Meier Estimate
• Middle Aged
• Neoadjuvant Therapy
• RNA, Messenger (genetics, metabolism)
• Sequence Analysis, DNA
• Up-Regulation