Sulphinpyrazone reduces endocardial injury and mural thrombosis.

Abstract

STUDY OBJECTIVE:

The aim of the study was to investigate the effectiveness of sulphinpyrazone, a drug which stabilises endothelial cell membranes, in reducing endocardial injury and mural thrombosis produced by lactic acid in the left ventricle.

DESIGN:

The left ventricular endocardium of isolated beating rat hearts, perfused via the aorta with oxygenated Krebs-Henseleit buffer, was exposed for up to 4 h to additional lactic acid (pH 6.4), with and without sulphinpyrazone (100 ng x ml-1). After flushing with buffer, passage of 10 ml blood, and further flushing, the hearts were fixed by coronary perfusion and the endocardium examined by scanning electron microscopy.

EXPERIMENTAL MATERIAL:

Hearts from 48 male albino Wistar rats, weight 270-380 g, were used.

MAIN RESULTS:

Morphometric analysis of the surface of the papillary muscles showed that lactic acid caused membrane injury in endothelial cells, up to 30% of which exfoliated. However when sulphinpyrazone was present, endothelial cell damage was reduced and there was up to 75% reduction in the area of exposed basal lamina or connective tissue. This was associated with a corresponding reduction in the extent of platelet adhesion (79%) and thrombus formation (94%).

CONCLUSIONS:

The results show that sulphinpyrazone has the potential to reduce the risk of mural thrombosis following endocardial injury.

• Authors: G Carter, J B Gavin
• Journal: Cardiovascular research (Cardiovasc Res) Vol. 24 Issue 4 Pg. 257-62 (Apr 1990) ISSN: 0008-6363 [Print] England
• PMID: 2346961 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lactates
• Sulfinpyrazone

Topics

• Animals
• Endocardium (drug effects, ultrastructure)
• Endothelium (ultrastructure)
• Heart Diseases (pathology, prevention & control)
• Lactates (toxicity)
• Male
• Microscopy, Electron, Scanning
• Myocardium (ultrastructure)
• Platelet Adhesiveness (drug effects)
• Rats
• Rats, Inbred Strains
• Sulfinpyrazone (therapeutic use)
• Thrombosis (pathology, prevention & control)
• Time Factors