Serum microRNAs levels in primary focal segmental glomerulosclerosis.

Abstract

BACKGROUND:

MicroRNAs (miRNAs, miRs) are involved in most physiological, developmental, and pathological processes. miR-192 and miR-205 are expressed preferentially in the renal cortex and closely relevant to the renal cell biology. In the present study, we aim to measure the serum levels of miR-192 and miR-205 and their correlation with clinicopathological data in patients with primary focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD).

METHODS:

Fifty-six patients (35 male, 21 female) with idiopathic nephrotic syndrome (FSGS 30, MCD 26) and 20 healthy controls were enrolled in the study. We quantified the serum levels of miR-192 and miR-205 in patients with FSGS and MCD by RT-qPCR.

RESULTS:

Patients with FSGS had higher serum levels of miR-192 and miR-205 than those with MCD (324.49 ± 42.74 fmol/l versus 90.19 ± 27.14 fmol/l, p < 0.01, 2.25 ± 0.69 fmol/l versus 0.60 ± 0.51 fmol/l, p < 0.01, respectively). The level of miR-192 was positively correlated with the proteinuria in patients with FSGS and MCD (r = 0.62, p < 0.001, r = 0.84, p < 0.001, respectively). Similarly, the level of miR-205 was positively correlated with the proteinuria in patients with FSGS (r = 0.54, p = 0.002). In addition, the serum level of miR-192 was significantly correlated with the degree of interstitial fibrosis in patients with FSGS (r = 0.342, p < 0.05).

CONCLUSIONS:

miR-192 and miR-205 have the potential as markers to differentiate FSGS from MCD.

• Authors: Xiaoyi Cai, Zhengkun Xia, Chunni Zhang, Yang Luo, Yuanfu Gao, Zhongmin Fan, Mengyuan Liu, Ying Zhang
• Journal: Pediatric nephrology (Berlin, Germany) (Pediatr Nephrol) Vol. 28 Issue 9 Pg. 1797-801 (Sep 2013) ISSN: 1432-198X [Electronic] Germany
• PMID: 23463342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenal Cortex Hormones
• MIRN192 microRNA, human
• MIRN205 microRNA, human
• MicroRNAs

Topics

• Adrenal Cortex Hormones (therapeutic use)
• Child
• Female
• Fibrosis
• Glomerular Filtration Rate
• Glomerulosclerosis, Focal Segmental (metabolism, pathology)
• Humans
• Kidney Function Tests
• Male
• MicroRNAs (blood)
• Nephrosis, Lipoid (pathology)
• Polymerase Chain Reaction
• Treatment Outcome