Abstract |
The stress response involves the activation of two corticotropin-releasing factor ( CRF) receptors types 1 and 2. The pituitary type 1 CRF receptors represent the primary receptors to activate the hypothalamic-pituitary-adrenocortical axis and, consequently, glucocorticoid production. Exogenous CRF induces an increase in glucocorticoid production and may protect the gastric mucosa against stress-induced injury. Here we examined contribution of glucocorticoids and CRF receptors type 2 to gastroprotective effect of exogenous CRF. Gastric injury was induced by 3 him-mobilization ( at 10 degrees C) in conscious rats or 3.5 h gastric ischemia-reperfusion in anaesthetized rats. Intraperitoneal administration of CRF at the doses of 1.25 or 2.5 Mg/kg increased plasma corticosterone levels and suppressed the occurrence of gastric erosion induced by each stimulus. Metyrapone injected before CRF caused an inhibition of CRF-induced corticosterone response and prevented the protective effect of CRF on the gastric mucosa against erosion caused by immobilization ( at 10 degrees C). However, metyrapone injection did not influence the protective effect of CRF on the gastric mucosa against ischemia-reperfusion-induced lesion. The protective effect of CRF on the gastric mucosa against ischemia-reperfusion-induced lesion was prevented by the nonselective CRF receptor antagonist astressin and selective type 2 CRF receptor antagonist astressin2-B. The results obtained suggest that exogenous CRF may protect the gastric mucosa against injury through involvement of glucocorticoids and also through CRF receptors type 2.
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Authors | L P Filaretova, T R Bagaeva, O Iu Morozova |
Journal | Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova
(Ross Fiziol Zh Im I M Sechenova)
Vol. 98
Issue 12
Pg. 1555-66
(Dec 2012)
ISSN: 0869-8139 [Print] Russia (Federation) |
PMID | 23461199
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimetabolites
- CRF receptor type 2
- Glucocorticoids
- Peptide Fragments
- Receptors, Corticotropin-Releasing Hormone
- astressin
- CRF receptor type 1
- Corticotropin-Releasing Hormone
- Corticosterone
- Metyrapone
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Topics |
- Animals
- Antimetabolites
(pharmacology)
- Cold Temperature
(adverse effects)
- Corticosterone
(blood)
- Corticotropin-Releasing Hormone
(pharmacology, therapeutic use)
- Gastric Mucosa
(drug effects, metabolism, pathology)
- Glucocorticoids
(pharmacology, therapeutic use)
- Immobilization
(adverse effects)
- Injections, Intraperitoneal
- Male
- Metyrapone
(pharmacology)
- Peptide Fragments
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Receptors, Corticotropin-Releasing Hormone
(agonists, metabolism)
- Reperfusion Injury
(drug therapy, pathology)
- Stomach
(drug effects, pathology)
- Stomach Ulcer
(drug therapy, etiology, metabolism)
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