Age and meloxicam modify the response of the glutamate vesicular transporters (VGLUTs) after transient global cerebral ischemia in the rat brain.

Abstract

AIMS:

This study analyzes how age and inflammation modify the response of the vesicular glutamate transporters (VGLUTs), VGLUT1-3 to global brain ischemia/reperfusion (I/R) in brain areas with different I/R vulnerabilities.

RESULTS:

Global ischemia was induced in 3- and 18-month-old male Sprague-Dawley rats and CA1 and CA3 hippocampal areas, dentate gyrus and cerebral cortex of sham-operated and I/R animals were removed 48 h after insult. Real-time PCR analysis revealed that I/R challenge resulted in a significant decrease of the VGLUT mRNA levels in young animals. Western blot assays showed a lessened age-dependent response to the ischemic damage in VGLUT1 and VGLUT3, while VGLUT2 presented an age and structure-dependent response to challenge. The use of the anti-inflammatory agent meloxicam following challenge showed that COX2 inhibition promotes the expression of VGLUTs in both sham and injured animals, which results in a lessened response to I/R injury.

CONCLUSIONS:

VGLUT1 and VGLUT3 presented an age-dependent response to ischemic damage, while this VGLUT response was age both and structure-dependent. In addition, COX-2 inhibition resulted in an increase of VGLUT1 and VGLUT2 protein amounts both in sham and injured animals together with a lessening of the transporters' response to ischemia.

• Authors: Irene L Llorente, Diego Pérez-Rodríguez, Taiana C Burgin, José M Gonzalo-Orden, Beatriz Martínez-Villayandre, Arsenio Fernández-López
• Journal: Brain research bulletin (Brain Res Bull) Vol. 94 Pg. 90-7 (May 2013) ISSN: 1873-2747 [Electronic] United States
• PMID: 23458738 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Thiazines
• Thiazoles
• Vesicular Glutamate Transport Proteins
• Meloxicam

Topics

• Age Factors
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Blotting, Western
• Brain (drug effects, metabolism)
• Brain Ischemia (metabolism)
• Disease Models, Animal
• Male
• Meloxicam
• Rats
• Rats, Sprague-Dawley
• Real-Time Polymerase Chain Reaction
• Thiazines (pharmacology)
• Thiazoles (pharmacology)
• Vesicular Glutamate Transport Proteins (biosynthesis)