HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus.

Abstract
Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.
AuthorsByoung-Shik Shim, Jung-Ah Choi, Ho-Hyun Song, Sung-Moo Park, In Su Cheon, Ji-Eun Jang, Sun Je Woo, Chung Hwan Cho, Min-Suk Song, Hyemi Kim, Kyung Joo Song, Jae Myun Lee, Suhng Wook Kim, Dae Sub Song, Young Ki Choi, Jae-Ouk Kim, Huan Huu Nguyen, Dong Wook Kim, Young Yil Bahk, Cheol-Heui Yun, Man Ki Song
JournalJournal of microbiology (Seoul, Korea) (J Microbiol) Vol. 51 Issue 1 Pg. 130-5 (Feb 2013) ISSN: 1976-3794 [Electronic] Korea (South)
PMID23456722 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • H1N1 virus hemagglutinin
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Influenza Vaccines
  • Vaccines, Synthetic
  • Cholera Toxin
Topics
  • Adjuvants, Immunologic (administration & dosage, genetics)
  • Administration, Sublingual
  • Animals
  • Antibodies, Neutralizing (analysis, blood)
  • Antibodies, Viral (analysis, blood)
  • Cholera Toxin (administration & dosage, genetics)
  • Disease Models, Animal
  • Enzyme-Linked Immunospot Assay
  • Hemagglutination Inhibition Tests
  • Hemagglutinin Glycoproteins, Influenza Virus (genetics, immunology)
  • Immunity, Mucosal
  • Influenza A Virus, H1N1 Subtype (genetics, immunology)
  • Influenza Vaccines (administration & dosage, genetics, immunology)
  • Leukocytes, Mononuclear (immunology)
  • Lung (virology)
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections (prevention & control)
  • Serum (immunology)
  • Vaccines, Synthetic (administration & dosage, genetics, immunology)
  • Viral Load

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: