Abstract | PURPOSE: To characterize the relationship between superparamagnetic ferritin-bound iron and diffusion tensor scalars in vitro, and validate the results in vivo. MATERIALS AND METHODS: The in vitro model consisted of a series of 40-mL 1.1% agarose gels doped with ferritin covering and exceeding those concentrations normally found within healthy human gray matter. Additionally, regions of interest were placed in the caudate, putamen, and globus pallidus of 29 healthy volunteer subjects 19-80 years of age. Carr-Purcell-Meiboom-Gill sequence (CPMG) and diffusion tensor imaging (DTI) data were collected at 1.5 Tesla (T) and 3T in vitro, and at 1.5T in vivo. RESULTS: In vitro, linear relationships were observed between ferritin-bound iron concentration, R2 (1/T2 ) and 1/SNR. Eigenvalue repulsion with increasing R2 (decreasing SNR) was reflected in an artifactual increase of fractional anisotropy. In vivo, similar relationships were observed, with mean diffusivity also decreasing linearly with increasing R2 . Lambda 3 showed the strongest correlation with R2 both in vitro and in vivo. CONCLUSION:
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Authors | Aaron M Rulseh, Jiří Keller, Jaroslav Tintěra, Milan Kožíšek, Josef Vymazal |
Journal | Journal of magnetic resonance imaging : JMRI
(J Magn Reson Imaging)
Vol. 38
Issue 5
Pg. 1103-10
(Nov 2013)
ISSN: 1522-2586 [Electronic] United States |
PMID | 23440865
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Wiley Periodicals, Inc. |
Chemical References |
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Brain
(cytology, metabolism)
- Diffusion Tensor Imaging
(instrumentation, methods)
- Female
- Ferritins
(metabolism)
- Humans
- In Vitro Techniques
- Iron
(metabolism)
- Male
- Middle Aged
- Neurons
(cytology, metabolism)
- Phantoms, Imaging
- Reproducibility of Results
- Sensitivity and Specificity
- Young Adult
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