Abstract | BACKGROUND:
Surfactant protein D ( SP-D) is a collection that plays important roles in modulating host defense functions and maintaining phospholipid homeostasis in the lung. The aim of current study was to characterize comparatively the SP-D response in bronchoalveolar lavage (BAL) and serum in three murine models of lung injury, using a validated ELISA technology for estimation of SP-D levels. METHODS: RESULTS: In lipopolysaccharide-challenged mice, the level of SP-D in BAL increased within 6 h, peaked at 51 h (4,518 ng/ml), and returned to base level at 99 h (612 ng/ml). Serum levels of SP-D increased immediately (8.6 ng/ml), peaked at 51 h (16 ng/ml), and returned to base levels at 99 h (3.8 ng/ml). In a subacute bleomycin inflammation model, SP-D levels were 4,625 and 367 ng/ml in BAL and serum, respectively, 8 days after exposure. In a chronic Pc inflammation model, the highest level of SP-D was observed 6 weeks after inoculation, with BAL and serum levels of 1,868 and 335 ng/ml, respectively. CONCLUSIONS: We conclude that serum levels of SP-D increase during lung injury, with a sustained increment during chronic inflammation compared with acute inflammation. A quick upregulation of SP-D in serum in response to acute airway inflammation supports the notion that SP-D translocates from the airways into the vascular system, in favor of being synthesized systemically. The study also confirms the concept of using increased SP-D serum levels as a biomarker of especially chronic airway inflammation.
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Authors | Maria Quisgaard Gaunsbaek, Karina Juhl Rasmussen, Michael F Beers, Elena N Atochina-Vasserman, Soren Hansen |
Journal | Lung
(Lung)
Vol. 191
Issue 3
Pg. 295-303
(Jun 2013)
ISSN: 1432-1750 [Electronic] United States |
PMID | 23435873
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipopolysaccharides
- Pulmonary Surfactant-Associated Protein D
- Bleomycin
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Topics |
- Acute Lung Injury
(blood, chemically induced, metabolism, microbiology)
- Animals
- Bleomycin
- Bronchoalveolar Lavage Fluid
(chemistry)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Female
- Immunohistochemistry
- Lipopolysaccharides
- Lung
(metabolism, microbiology)
- Lung Injury
(blood, chemically induced, metabolism, microbiology)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Nude
- Pneumocystis carinii
- Pneumonia, Pneumocystis
(blood, metabolism, microbiology)
- Pulmonary Surfactant-Associated Protein D
(blood, metabolism)
- Time Factors
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