HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Overexpression of initiator methionine tRNA leads to global reprogramming of tRNA expression and increased proliferation in human epithelial cells.

Abstract
Transfer RNAs (tRNAs) are typically considered housekeeping products with little regulatory function. However, several studies over the past 10 years have linked tRNA misregulation to cancer. We have previously reported that tRNA levels are significantly elevated in breast cancer and multiple myeloma cells. To further investigate the cellular and physiological effects of tRNA overexpression, we overexpressed tRNAi(Met) in two human breast epithelial cell lines. We then determined tRNA abundance changes and performed phenotypic characterization. Overexpression of tRNAi(Met) significantly altered the global tRNA expression profile and resulted in increased cell metabolic activity and cell proliferation. Our results extend the relevance of tRNA overexpression in human cells and underscore the complexity of cellular regulation of tRNA expression.
AuthorsMariana Pavon-Eternod, Suzana Gomes, Marsha R Rosner, Tao Pan
JournalRNA (New York, N.Y.) (RNA) Vol. 19 Issue 4 Pg. 461-6 (Apr 2013) ISSN: 1469-9001 [Electronic] United States
PMID23431330 (Publication Type: Letter, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Transfer, Amino Acyl
  • tRNA(m)(Met), methionine-
  • RNA, Transfer
Topics
  • Breast Neoplasms (metabolism, pathology)
  • Cell Line, Tumor
  • Cell Proliferation
  • Humans
  • Peptide Chain Initiation, Translational
  • Protein Biosynthesis
  • RNA, Transfer (genetics)
  • RNA, Transfer, Amino Acyl (genetics)
  • Transcriptome

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: