Correlation of MAGE-A tumor antigens and the efficacy of various chemotherapeutic agents in head and neck carcinoma cells.

Abstract

OBJECTIVES:

The present study examined the relationship between MAGE-A tumor antigens and the efficacy of diamindichloridoplatin (DDP), 5-fluorouracil (5-FU), docetaxel, and paclitaxel for in vitro treatment of head and neck cancer.

METHODS:

In the present study, five cell lines of human squamous cell carcinomas were treated with DDP (25-400 μM), 5-FU (0.75-12 mM), docetaxel (1.56-25 nM), and paclitaxel (1.56-25 nM) for a period of 24 or 48 h. The efficacy of the agents was observed dynamically using real-time cell analysis. Subsequently, the expression levels of MAGE-A1, MAGE-A5, MAGE-A8, MAGE-A9, MAGE-A11, and MAGE-A12 were determined by quantitative real-time polymerase chain reaction. Chemosensitivity and MAGE-A-expression were correlated by linear regression.

RESULTS:

The tumor cell lines showed a highly differentiated response to the chemotherapeutic agents. Expression of MAGE-A11 was significantly associated with a poorer response to treatment with DDP, 5-FU, docetaxel, and paclitaxel. Two cell lines, one of which was MAGE-A11-positive, showed a significant and concentration-dependent cisplatin-induced growth spurt during the first 24 h after treatment. MAGE-A5 was connected to a positive effect on treatment with paclitaxel within the first 24 h after application. In association with docetaxel treatment, MAGE-A8 was connected to a poorer susceptibility.

CONCLUSIONS:

The results describe, for the first time, a correlation between these MAGE-A tumor antigens and the susceptibility of head and neck cancer cells to DDP, 5-FU, docetaxel, and paclitaxel.

CLINICAL RELEVANCE:

These findings could affect the antineoplastic treatment of patients with MAGE-A11-positive tumors.

• Authors: S Hartmann, U Kriegebaum, N Küchler, R C Brands, C Linz, A C Kübler, U D A Müller-Richter
• Journal: Clinical oral investigations (Clin Oral Investig) Vol. 18 Issue 1 Pg. 189-97 (Jan 2014) ISSN: 1436-3771 [Electronic] Germany
• PMID: 23430338 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Antineoplastic Agents
• DNA Primers

Topics

• Antigens, Neoplasm (immunology)
• Antineoplastic Agents (therapeutic use)
• Base Sequence
• Carcinoma, Squamous Cell (drug therapy, immunology, pathology)
• Cell Line, Tumor
• DNA Primers
• Head and Neck Neoplasms (drug therapy, immunology, pathology)
• Humans
• Reverse Transcriptase Polymerase Chain Reaction