Abstract | BACKGROUND: Severe acute pancreatitis (SAP) is a serious systemic disease with a sustained high mortality rate. Extensive evidence has shown that gut barrier dysfunction plays a critical role in the pathophysiology of SAP. AIM: Investigating the role of intestinal mucosa oxidative stress in gut barrier dysfunction of SAP. MATERIALS AND METHODS: RESULTS: At four and eight hours after SAP induction, the SAP group mice had significantly higher pancreatic and gut pathological scores (p < 0.01) and increased serum levels of amylase (p < 0.05), DAO and TNF-α (p < 0.01) and increased MDA contents and XO activity of gut mucosa (p < 0.01) compared with those of control mice. There were significantly lower GSH contents (p < 0.05) and SOD activity (p < 0.01) of gut mucosa in the SAP mice. It was also observed that the gut mucosa cells of SAP mice had significantly higher caspase-3 activity and apoptosis index (p < 0.01). CONCLUSIONS: In SAP, waterfall-style release of inflammatory factors such as TNF-α led to ischemia-reperfusion injury of gut mucosa which resulted in serious oxidative stress and activation of caspase-3 pathway and severe apoptosis of gut mucosa. Therefore, intestinal mucosal oxidative stress may play an important role in the mechanism of gut barrier dysfunction.
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Authors | R Tian, J-T Tan, R-L Wang, H Xie, Y-B Qian, K-L Yu |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 17
Issue 3
Pg. 349-55
(Feb 2013)
ISSN: 1128-3602 [Print] Italy |
PMID | 23426538
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Inflammation Mediators
- Tumor Necrosis Factor-alpha
- Ceruletide
- Caspase 3
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Topics |
- Acute Disease
- Analysis of Variance
- Animals
- Apoptosis
- Caspase 3
(metabolism)
- Ceruletide
(toxicity)
- Disease Models, Animal
- In Situ Nick-End Labeling
- Inflammation Mediators
(metabolism)
- Intestinal Mucosa
(physiopathology)
- Male
- Mice
- Mice, Inbred BALB C
- Oxidative Stress
- Pancreatitis
(physiopathology)
- Random Allocation
- Reperfusion Injury
(physiopathology)
- Severity of Illness Index
- Tumor Necrosis Factor-alpha
(metabolism)
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