HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Redox-proteomic analysis of doxorubicin resistance-induced altered thiol activity in uterine carcinoma.

Abstract
Doxorubicin is an anticancer drug used in a wide range of cancer therapies; however, doxorubicin-induced drug resistance is one of the most serious obstacles of cancer chemotherapy. Recent studies have indicated that reduced oxidative stress levels in cancer cells induce drug resistance. However, the redox-modifications of resistance - associated cellular targets are largely unknown. Thus, the current study employed cysteine-labeling based two-dimensional differential gel electrophoresis (2D-DIGE) combined with MALDI-TOF mass spectrometry (MALDI-TOF MS) to analyze the effect of doxorubicin resistance on redox regulation in uterine cancer and showed 33 spots that were significantly changed in thiol reactivity. These proteins involve cytoskeleton regulation, signal transduction, redox-regulation, glycolysis, and cell-cycle regulation. The current work shows that the redox 2D-DIGE-based proteomic strategy provides a rapid method to study the molecular mechanisms of doxorubicin-induced drug resistance in uterine cancer. The identified targets may be used to further evaluate their roles in drug-resistance formation and for possible diagnostic or therapeutic applications.
AuthorsSzu-Ting Lin, Yi-Wen Lo, Shing-Jyh Chang, Wen-Ching Wang, Margaret Dah-Tsyr Chang, Ping-Chiang Lyu, Yi-Wen Chen, Hsiu-Chuan Chou, Hong-Lin Chan
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 78-79 Pg. 1-8 (May 05 2013) ISSN: 1873-264X [Electronic] England
PMID23425445 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier B.V. All rights reserved.
Chemical References
  • Antineoplastic Agents
  • Reactive Oxygen Species
  • Sulfhydryl Compounds
  • Doxorubicin
Topics
  • Antineoplastic Agents (therapeutic use)
  • Cell Line, Tumor
  • Doxorubicin (therapeutic use)
  • Drug Resistance, Neoplasm
  • Female
  • Humans
  • Oxidation-Reduction
  • Proteomics
  • Reactive Oxygen Species (metabolism)
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sulfhydryl Compounds (metabolism)
  • Uterine Neoplasms (drug therapy, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: