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Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life.

AbstractBACKGROUND:
Oral supplementation with probiotic bacteria can protect against the development of allergic and inflammatory diseases.
OBJECTIVE:
The aim of this study was to investigate potential immunomodulatory and allergy-protective effects of processed Lactobacillus rhamnosus GG (LGG)-derived supernatants early in life in neonatal mice.
METHODS:
In vitro, RAW264.7 mouse macrophages were stimulated with viable LGG, LGG-derived supernatants, prepared from different growth phases, and different size fractions thereof, and pro- and anti-inflammatory cytokine production was analysed. Supernatant fractions were also treated with protease, DNAse or carbohydrate-digesting enzymes to define the nature of immunomodulatory components. In vivo, neonatal Balb/c mice were orally supplemented with differentially processed LGG supernatants. Starting at 4 weeks of age, a protocol of ovalbumin-induced acute allergic airway inflammation was applied and protective effects of processed LGG supernatants were assessed.
RESULTS:
Incubation of RAW264.7 cells with LGG-derived supernatants significantly increased TNFα and IL-10 production. These effects were not restricted to a particular molecular size fraction. Treatment with protease, but not with DNAse or carbohydrate-digesting enzymes, completely abolished the immunomodulatory activities. Incubation of TLR/NOD-transfected cells with LGG-derived supernatants revealed that recognition and signalling of bioactive components is mediated by TLR2 and NOD2. In vivo supplementation of newborn mice with processed LGG-derived supernatants resulted in pronounced protective effects on the allergic inflammatory response as reflected by reduced eosinophil numbers, modified T helper cell cytokine production, significantly less lung inflammation and reduced goblet cell numbers in comparison with sham-treated controls.
CONCLUSION:
LGG-derived supernatants exert immunomodulatory activities, and neonatal administration of specifically processed supernatants may provide an alternative to viable probiotics in reducing allergic inflammatory responses.
AuthorsH Harb, E A F van Tol, H Heine, M Braaksma, G Gross, K Overkamp, M Hennen, M Alrifai, M L Conrad, H Renz, H Garn
JournalClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 43 Issue 3 Pg. 353-64 (Mar 2013) ISSN: 1365-2222 [Electronic] England
PMID23414544 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2012 Blackwell Publishing Ltd.
Chemical References
  • Culture Media, Conditioned
  • Immunologic Factors
  • Nod2 Signaling Adaptor Protein
  • Toll-Like Receptor 2
Topics
  • Animals
  • Cell Line
  • Culture Media, Conditioned (pharmacology)
  • Female
  • Humans
  • Hypersensitivity (immunology, metabolism, therapy)
  • Immunologic Factors (pharmacology)
  • Inflammation (immunology, metabolism, therapy)
  • Lacticaseibacillus rhamnosus (chemistry, growth & development, immunology)
  • Mice
  • Nod2 Signaling Adaptor Protein (metabolism)
  • Probiotics
  • Toll-Like Receptor 2 (metabolism)

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