Abstract |
EDDHSA/Fe is a promising substitute of EDDHA/Fe to fight iron chlorosis. o,o-EDDHSA structure contains two chiral carbons giving the racemic and meso couples of stereoisomers. Ion-pair HPLC and UHPLC-UV/Vis-ESI-MS/MS methods were developed for the determination of racemic and meso-o,o-EDDHSA/Fe in commercial samples of chelates. The lack of a commercial EDDHSA standard was overcome by sulfonation of a commercial available o,o- EDDHA standard and subsequent quantification by (1)H-NMR. Assignment of configurations was carried out starting from racemic and meso-o,o- EDDHA/Fe by direct sulfonation to give the corresponding o,o-EDDHSA/Fe isomers. The performances of these methods were assessed in terms of intra and inter-day precision, linearity and selectivity. The high selectivity and lower detection limit (nanomolar) of the UHPLC-ESI-MS/MS method could allow to deepen the knowledge relative to meso and rac-o,o-EDDHSA/Fe interactions with plants, its fate in different soil conditions, its mobility and other environmental aspects.
|
Authors | Alessandro Biasone, Giusto Cianci, Donata Di Tommaso, Alberto Piaggesi, Emilio Tagliavini, Paola Galletti, Fabio Moretti |
Journal | Journal of chromatography. A
(J Chromatogr A)
Vol. 1282
Pg. 142-52
(Mar 22 2013)
ISSN: 1873-3778 [Electronic] Netherlands |
PMID | 23411145
(Publication Type: Journal Article)
|
Copyright | Copyright © 2013 Elsevier B.V. All rights reserved. |
Chemical References |
- Acetonitriles
- Acids, Acyclic
- Ions
- N,N'-ethylenediamine-di-(2-hidroxy-5-sulfophenylacetic) acid
- Phenylacetates
- Iron
- Hydrochloric Acid
- acetonitrile
|
Topics |
- Acetonitriles
(chemistry)
- Acids, Acyclic
(chemistry)
- Chromatography, High Pressure Liquid
(methods)
- Hydrochloric Acid
(chemistry)
- Ions
(analysis, chemistry)
- Iron
(analysis, chemistry)
- Limit of Detection
- Phenylacetates
(analysis, chemistry)
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrometry, Mass, Electrospray Ionization
- Stereoisomerism
- Tandem Mass Spectrometry
(methods)
|