The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity.

Abstract

The saponin monomer of dwarf lilyturf tuber, DT-13, exhibits anticancer activity by reducing human breast cancer cell adhesion and migration under hypoxia. To further investigate the anticancer activity of DT-13, we investigated whether DT-13 exhibits anti-angiogenic activity. DT-13 showed no effect on human umbilical vein endothelial cell proliferation but inhibited tube formation and migration under normoxia and hypoxia. Moreover, DT-13 significantly reduced density of vessels in vivo observed from a chicken chorioallantoic membrane model. Western blotting results showed that DT-13 suppressed the increased level of hypoxia-inducible factor 1α, p-extracellular signal-regulated kinase 1/2 and p-Akt induced by hypoxia. Enzyme-linked immunosorbent assay revealed that vascular endothelial growth factor excretion was suppressed by DT-13. DT-13 inhibited migration and tube formation induced by vascular endothelial growth factor under normoxia and hypoxia. In addition, DT-13 reduced the level of p-vascular endothelial growth factor receptor 2 and p-Akt induced by vascular endothelial growth factor. Our data suggest that DT-13 inhibits angiogenesis under normoxia and hypoxia and also inhibits angiogenesis induced by vascular endothelial growth factor via targeting at multi elements.

• Authors: Renping Zhao, Li Sun, Sensen Lin, Xianshu Bai, Boyang Yu, Shengtao Yuan, Luyong Zhang
• Journal: Oncology reports (Oncol Rep) Vol. 29 Issue 4 Pg. 1379-86 (Apr 2013) ISSN: 1791-2431 [Electronic] Greece
• PMID: 23404207 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hypoxia-Inducible Factor 1, alpha Subunit
• Saponins
• Vascular Endothelial Growth Factor A

Topics

• Animals
• Breast Neoplasms (genetics, pathology)
• Cell Adhesion (drug effects)
• Cell Hypoxia (drug effects, genetics)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Chickens
• Chorioallantoic Membrane (drug effects)
• Female
• Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (genetics, metabolism)
• Liriope Plant (chemistry)
• Neovascularization, Pathologic (drug therapy, genetics, pathology)
• Saponins (chemistry, pharmacology)
• Signal Transduction (drug effects)
• Vascular Endothelial Growth Factor A (metabolism)