Isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria.

The objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (SPB) from raw animal materials, and to develop a SPB-specific bacteriophage cocktail for rendering application. Meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate SPB (n = 142). Bacteriophages (n = 52) specific to SPB were isolated and purified from the above samples using 18 of those isolated SPB strains as hosts. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia coli, Citrobacter freundii, and Hafnia alvei) were determined. Electron microscopy observation of 9 phages selected for the phage cocktail revealed that 6 phages belonged to the family of Siphoviridae and 3 belonged to the Myoviridae family. Restriction enzyme digestion analysis with endonuclease DraI detected 6 distinguished patterns among the 9 phages. Phage treatment prevented the growth of SPB for up to 10 h with multiplicity of infection ratios of 1, 10, 100, and 1000 in tryptic soy broth at 30 °C, and extended the lag phase of SPB growth for 2 h at 22 °C with multiplicities of infection of 10, 100, and 1000. These results suggest that the selected bacteriophage cocktail has a high potential for phage application to control SPB in raw animal materials destined for the rendering process.
AuthorsChao Gong, Spencer Heringa, Randhir Singh, Jinkyung Kim, Xiuping Jiang
JournalCanadian journal of microbiology (Can J Microbiol) Vol. 59 Issue 1 Pg. 39-45 (Jan 2013) ISSN: 1480-3275 [Electronic] Canada
PMID23391228 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
  • Bacteriophages (classification, genetics, isolation & purification, physiology, ultrastructure)
  • Gram-Negative Aerobic Rods and Cocci (virology)
  • Host Specificity
  • Microscopy, Electron, Transmission
  • Myoviridae (classification, genetics, ultrastructure)
  • Restriction Mapping
  • Siphoviridae (classification, genetics, ultrastructure)

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