Potential of urinary metabolites for diagnosing multiple sclerosis.

Abstract

A definitive diagnostic test for multiple sclerosis (MS) does not exist; instead physicians use a combination of medical history, magnetic resonance imaging, and cerebrospinal fluid analysis (CSF). Significant effort has been employed to identify biomarkers from CSF to facilitate MS diagnosis; however, none of the proposed biomarkers have been successful to date. Urine is a proven source of metabolite biomarkers and has the potential to be a rapid, noninvasive, inexpensive, and efficient diagnostic tool for various human diseases. Nevertheless, urinary metabolites have not been extensively explored as a source of biomarkers for MS. We demonstrate that urinary metabolites have significant promise for monitoring disease-progression, and response to treatment in MS patients. NMR analysis of urine permitted the identification of metabolites that differentiate experimental autoimmune encephalomyelitis (EAE)-mice (prototypic disease model for MS) from healthy and MS drug-treated EAE mice.

• Authors: Teklab Gebregiworgis, Chandirasegaran Massilamany, Arunakumar Gangaplara, Sivasubramani Thulasingam, Venkata Kolli, Mark T Werth, Eric D Dodds, David Steffen, Jay Reddy, Robert Powers
• Journal: ACS chemical biology (ACS Chem Biol) Vol. 8 Issue 4 Pg. 684-90 (Apr 19 2013) ISSN: 1554-8937 [Electronic] United States
• PMID: 23369377 (Publication Type: Letter, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers

Topics

• Amino Acid Sequence
• Animals
• Biomarkers (urine)
• Disease Models, Animal
• Female
• Magnetic Resonance Spectroscopy
• Mice
• Mice, Inbred C57BL
• Molecular Sequence Data
• Multiple Sclerosis (diagnosis, urine)