Abstract | OBJECTIVE: ANIMALS: 9 healthy adult Lacaune ewes. PROCEDURES: Animals received an IM and SC administration of florfenicol (20 mg/kg) in a 2-way crossover design. Samples of blood and tear fluid were collected before and for 24 hours after administration. Concentrations of florfenicol in plasma and tear fluid were measured via high-performance liquid chromatography. The MIC of florfenicol for various Mycoplasma strains cultured from sheep and goats was determined via an agar dilution method. RESULTS: Mean florfenicol concentration in tear fluid for the 24-hour period was significantly higher after IM administration (0.70 μg/mL) than after SC administration (0.22 μg/mL) and was maintained for a longer duration. The lacrimal fluid-to-plasma concentration ratio was not different between the 2 routes of administration, with mean values of 40.2% and 32.5% after IM and SC administration, respectively. The MIC for Mycoplasma agalactiae, Mycoplasma conjunctivae, and Mycoplasma mycoides isolates ranged from 0.5 to 8 μg of florfenicol/mL. Two strains of M agalactiae could be considered resistant to florfenicol. CONCLUSIONS AND CLINICAL RELEVANCE:
Florfenicol readily penetrated the preocular tear fluid of sheep after IM and SC administration. For both routes of administration, doses > 20 mg/kg would be necessary to achieve tear fluid concentrations of florfenicol greater than the MICs for most strains of Mycoplasma organisms.
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Authors | Alain Regnier, Valérie Laroute, Anne Gautier-Bouchardon, Véronique Gayrard, Nicole Picard-Hagen, Pierre-Louis Toutain |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 74
Issue 2
Pg. 268-74
(Feb 2013)
ISSN: 1943-5681 [Electronic] United States |
PMID | 23363353
(Publication Type: Journal Article, Randomized Controlled Trial)
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Chemical References |
- Anti-Bacterial Agents
- florfenicol
- Thiamphenicol
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Topics |
- Animals
- Anti-Bacterial Agents
(administration & dosage, analysis, pharmacology)
- Chromatography, High Pressure Liquid
(veterinary)
- Cross-Over Studies
- Female
- Injections, Intramuscular
(veterinary)
- Injections, Subcutaneous
(veterinary)
- Keratoconjunctivitis, Infectious
(drug therapy)
- Microbial Sensitivity Tests
- Mycoplasma
(drug effects)
- Mycoplasma Infections
(drug therapy, microbiology)
- Sheep
- Sheep Diseases
(drug therapy)
- Species Specificity
- Tears
(chemistry)
- Thiamphenicol
(administration & dosage, analogs & derivatives, analysis, pharmacology)
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